Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, 100C, 20 minutes
Permeabilization
No
Other product details
Dilution
1/80
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse/rabbit IgG containing 10% animal serum
Host species: Mouse
Clonality: Monoclonal
Conjugation: HRP polymer
Host species: Mouse
Clonality: Monoclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Various retrieval protocols (proteinase K enzyme, citrate, EDTA,no ptt) citrate at 100C-steamer for 20 minutes gave best results with polymer detection (Leica Microsystems Refine) with DAB and hematoxylin counterstain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Ronald Houston
Verified customer
Submitted Apr 10 2009