Overview

  • Product name

    Anti-C5 antibody [12F3]
    See all C5 primary antibodies
  • Description

    Mouse monoclonal [12F3] to C5
  • Host species

    Mouse
  • Specificity

    ab17457 reacts with C5 in normal human plasma and no reaction is seen with plasma from C5 deficient patients.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native C5 protein isolated from human plasma.

  • Epitope

    Epitope specificity differs from that of ab17930 but slightly overlaps, as determined by inhibition ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab17457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/6000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 190 kDa. ab17457 recognises C5 in non reduced form only by western blot.


    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
      Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
    • Involvement in disease

      Defects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
      Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
    • Sequence similarities

      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Anaphylatoxin C5a analog antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
      • C5 antibody
      • C5a anaphylatoxin antibody
      • C5a antibody
      • C5b antibody
      • CO5_HUMAN antibody
      • Complement C5 alpha'' chain antibody
      • Complement C5 antibody
      • Complement component C5 antibody
      • CPAMD4 antibody
      • prepro-C5 antibody
      see all

    References

    ab17457 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for your enquiry. For ab11898, the antibody should recognize the whole mouse C5 chain; since this is a polyclonal antibody it is very likely that it recognizes both C5a and C5b. As the sequence of the immunogen used is commercially sensitive, we can't guarantee this however. For ab17930 and ab17457, the antibodies only recognize non-reduced C5 where the molecule is not split into its component subunit chains. The antibodies are expected to recognize an epitope on the interface between the different subunits, though that is purely speculation. All three antibodies are £ 198.00. Please contact us again if you have any additional questions.

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