Overview

  • Product name

  • Description

    Rabbit polyclonal to C5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human C5 aa 650-750 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab92654)

  • Positive control

    • This antibody gave a positive signal in Human Plasma (Total Protein).

Properties

Applications

Our Abpromise guarantee covers the use of ab66854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 188 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
      Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
    • Involvement in disease

      Defects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
      Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
    • Sequence similarities

      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Anaphylatoxin C5a analog antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
      • C5 antibody
      • C5a anaphylatoxin antibody
      • C5a antibody
      • C5b antibody
      • CO5_HUMAN antibody
      • Complement C5 alpha'' chain antibody
      • Complement C5 antibody
      • Complement component C5 antibody
      • CPAMD4 antibody
      • prepro-C5 antibody
      see all

    Images

    • Anti-C5 antibody (ab66854) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 188 kDa
      Observed band size: 180 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 238 kDa, 68 kDa. We are unsure as to the identity of these extra bands.

    References

    ab66854 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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