Anti-C5 / C5a antibody [557] (ab11876)
Key features and details
- Mouse monoclonal [557] to C5 / C5a
- Suitable for: WB, Inhibition Assay, Functional Studies, ELISA
- Reacts with: Human
- Isotype: IgG2a
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-C5 / C5a antibody [557]
See all C5 / C5a primary antibodies -
Description
Mouse monoclonal [557] to C5 / C5a -
Host species
Mouse -
Specificity
This antibody reacts with an epitope on C5 and C5a. The antibody 557 is capable to inhibit the binding of C5a to the C5a receptor through a competitive mechanism, it does not block the cleavage of C5 into C5a and C5b. -
Tested applications
Suitable for: WB, Inhibition Assay, Functional Studies, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type corresponding to Human C5/ C5a.
Database link: P01031 -
Epitope
Ab11876 recognizes an epitope located within the alpha-chain of C5 that corresponds to C5a. -
Positive control
- Recombinant C5
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
0.2 µm filtered -
Clonality
Monoclonal -
Clone number
557 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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Inhibition Assay |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
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ELISA | (1) |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
Inhibition Assay
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). -
Involvement in disease
Complement component 5 deficiency
An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705). -
Sequence similarities
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 727 Human
- Omim: 120900 Human
- SwissProt: P01031 Human
- Unigene: 494997 Human
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Alternative names
- Anaphylatoxin C5a analog antibody
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
- C5 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab11876 has been referenced in 6 publications.
- Garcia-Arguinzonis M et al. Alternative C3 Complement System: Lipids and Atherosclerosis. Int J Mol Sci 22:N/A (2021). PubMed: 34066088
- Peng L et al. A Novel Cleavage Pattern of Complement C5 Induced by Chlamydia trachomatis Infection via the Chlamydial Protease CPAF. Front Cell Infect Microbiol 11:732163 (2021). PubMed: 35087765
- Yuan K et al. Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression. J Exp Clin Cancer Res 39:9 (2020). PubMed: 31928530
- Lu W et al. C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization. Mol Med Rep 18:2110-2116 (2018). PubMed: 29956782
- Wangdi T et al. The Vi capsular polysaccharide enables Salmonella enterica serovar typhi to evade microbe-guided neutrophil chemotaxis. PLoS Pathog 10:e1004306 (2014). Mouse, Human . PubMed: 25101794
- Kola A et al. Epitope mapping of a C5a neutralizing mAb using a combined approach of phage display, synthetic peptides and site-directed mutagenesis. Immunotechnology 2:115-26 (1996). PubMed: 9373320