Anti-C5a antibody [7L11] (ab194637)
Key features and details
- Rat monoclonal [7L11] to C5a
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-C5a antibody [7L11]
See all C5a primary antibodies -
Description
Rat monoclonal [7L11] to C5a -
Host species
Rat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse C5a aa 650-800.
Database link: P06684 -
Positive control
- IHC-P: Mouse kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Add 200 µl sterile PBS. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
7L11 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 115 kDa.
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IHC-P |
1/100 - 1/200.
Antigen retrieval: PK (10 µg/ml). |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 115 kDa. |
IHC-P
1/100 - 1/200. Antigen retrieval: PK (10 µg/ml). |
Target
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Relevance
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Images
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Immunohistochemical analysis of paraffin-embedded sections of mouse kidney tissue samples from the folic acid-induced kidney injury model labelling C5a with AB194637 at 1/100-200 dilution. The samples were fixed using 4% PFA at 4°C overnight. A 4 µm section was subjected to IHC-P.
Antigen retrieval: PK (10 µg/ml).
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Anti-C5a antibody [7L11] (ab194637) + Mouse serum C5a
Predicted band size: 115 kDa
Datasheets and documents
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Datasheet download
References (4)
ab194637 has been referenced in 4 publications.
- Li C et al. Xuanfei Baidu Decoction suppresses complement overactivation and ameliorates IgG immune complex-induced acute lung injury by inhibiting JAK2/STAT3/SOCS3 and NF-κB signaling pathway. Phytomedicine 109:154551 (2023). PubMed: 36610119
- Li X et al. Deficiency of Mouse FHR-1 Homolog, FHR-E, Accelerates Sepsis, and Acute Kidney Injury Through Enhancing the LPS-Induced Alternative Complement Pathway. Front Immunol 11:1123 (2020). PubMed: 32636836
- Wang B et al. Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia-reperfusion. Mol Med Rep 18:4523-4529 (2018). PubMed: 30221740
- Feng F et al. PVT1 regulates inflammation and cardiac function via the MAPK/NF-?B pathway in a sepsis model. Exp Ther Med 16:4471-4478 (2018). PubMed: 30546393