Product nameAnti-C5a antibody [EPR19699-24]
See all C5a primary antibodies
DescriptionRabbit monoclonal [EPR19699-24] to C5a
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human C5a aa 200-1100. The exact sequence is proprietary.
Database link: P01031
- WB: Human serum. IP: Human serum.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab202039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Anti-C5a antibody [EPR19699-24] (ab202039) at 1/1000 dilution + Human serum at 20 µg
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Exposure time: 3 minutes
C5 is cleaved into C5α and C5β and this antibody recognizes the C5α. The molecular weight observed is consistent with what has been described in the literature (PMID: 23401592)
C5a was immunoprecipitated from 0.35 mg human serum 10μg with ab202039 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab202039 at 1/2000 dilution (0.26 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/10,000 dilution.
Lane 1: Human serum 10μg.
Lane 2: ab202039 IP in human serum.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202039 in human serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This antibody recognizes C5a.
ab202039 has not yet been referenced specifically in any publications.