Overview

  • Product name
    Anti-C5a / C5a des Arg antibody [2942]
    See all C5a / C5a des Arg primary antibodies
  • Description
    Mouse monoclonal [2942] to C5a / C5a des Arg
  • Host species
    Mouse
  • Specificity
    This antibody reacts with a neo-epitope on C5a that is formed upon cleavage of C5 into C5a and C5b. It will detect C5a and C5a des-Arg (Not C5).
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, Functional Studies, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control
    • Eye tissue of patient with age-related macular degeneration AMD IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab11877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Relevance
    C5 is involved in the activation of the lytic pathway within the complement system, which is an important factor in innate immunity.
  • Database links
  • Alternative names
    • Anaphylatoxin C5a analog antibody
    • C5 antibody
    • C5a anaphylatoxin antibody
    • Complement C5 precursor antibody
    • Complement component 5 antibody
    • Complement component C5a antibody
    • CPAMD 4 antibody
    • CPAMD4 antibody
    • MGC142298 antibody
    see all

Images

  • ICC/IF image of ab11877 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11877, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab11877 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11877, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Kajikawa T  et al. Safety and Efficacy of the Complement Inhibitor AMY-101 in a Natural Model of Periodontitis in Non-human Primates. Mol Ther Methods Clin Dev 6:207-215 (2017). IHC-P ; Cynomolgus monkey . Read more (PubMed: 28879212) »
  • Nozaki M  et al. Drusen complement components C3a and C5a promote choroidal neovascularization. Proc Natl Acad Sci U S A 103:2328-33 (2006). Read more (PubMed: 16452172) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us.

Unfortunately ab11877 has not been tested with bovine samples, and, according to our records it is not very likely it will recognise the protein from this species.

In which application would you be interested in using the antibody?

I can try to find out the percentage of homology between the immunogen of any of our anti-C5 antibodies and the bovine protein.

In case there is >85% of homology with any of them, you can participate in our Abreview system, by which you submit the results from testing the antibody in the new species, and beneficiate from a free antibody in compensation.

I look forward to receiving your reply. Do not hesitate to contact us for further assistance.

Read More

Question
Answer

We think the chance that this antibody will recognize bovine C5a is small.  Please let me know if you have any further questions.

Read More

Answer

Thank you for your enquiry. C5a is a 74 amino acids peptide with a molecular weight of 11.2 kDa. If you have any additional questions, please contact us again.

Read More

Question
Answer

Thank you for your enquiry. C5 is a 31 kDa protein and the antibody will react with C5. The following reference should be helpful for you: Referring to the article of Claudia Link (Selection of phage-displayed anti-guinea pig C5 or C5a antibodies and their application in xenotransplantation. Molecular Immunology 36 (1999) 1235-1247) a band of about 32 kDa in human sera seems to be normal. If you have any additional questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up