Key features and details
- Rabbit polyclonal to C5a-R
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C5a-R antibody
See all C5a-R primary antibodies
DescriptionRabbit polyclonal to C5a-R
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
- WB: PC-3, HepG2 and HEK-293 whole cell lysates. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab234757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 39 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionReceptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsSulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
Cellular localizationCell membrane.
- Information by UniProt
- ANAPHYLATOXIN RECEPTOR antibody
- C5a anaphylatoxin chemotactic receptor antibody
- C5a anaphylatoxin receptor antibody
All lanes : Anti-C5a-R antibody (ab234757) at 1/1000 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000000 dilution
Predicted band size: 39 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for C5a-R (green) using ab234757 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488 conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234757 has not yet been referenced specifically in any publications.