RecombinantRabMAb

Recombinant Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

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Flow Cytometry - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
  • Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
  • Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23278-117] to C5a-R - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Unconjugated

Overview

  • Product name

    Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free
    See all C5a-R primary antibodies

  • Description

    Rabbit monoclonal [EPR23278-117] to C5a-R - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human spleen and lung tissue. Clear cell carcinoma of human kidney tissue. ICC/IF: Human PBMCs. Flow Cyt: Human peripheral blood mononuclear cells.

  • General notes

    ab272400 is the carrier-free version of ab252435.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
Application notes
Is unsuitable for IP or WB.

Target

  • Function

    Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Post-translational
    modifications

    Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANAPHYLATOXIN RECEPTOR antibody
      • C5a anaphylatoxin chemotactic receptor antibody
      • C5a anaphylatoxin receptor antibody
      • C5A antibody
      • C5a ligand antibody
      • C5a-R antibody
      • C5aR antibody
      • C5AR_HUMAN antibody
      • c5ar1 antibody
      • C5R1 antibody
      • CD88 antibody
      • CD88 antigen antibody
      • Complement component 5 receptor 1 antibody
      • Complement component 5 receptor 2 antibody
      • Complement component 5a receptor 1 antibody
      see all

    Images

    • Flow Cytometry - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
      Flow Cytometry - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

      Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling C5a-R with ab252435 at 1/500 dilution (0.1µg)/ Right panel, compared with a Rabbit monoclonal IgG (ab172730) / Left panel, isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab252435 (Right). Then stained with anti-CD11b conjugated to BV421. Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252435).

    • Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
      Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMCs labelling C5a-R with ab252435 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplamic and membranous staining in subsets of human PBMC. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 µg/ml dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252435).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

      Immunohistochemical analysis of paraffin-embedded clear cell carcinoma of human kidney tissue labeling C5a-R with ab252435 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on scattered cells in clear cell carcinoma of human kidney is observed. The section was incubated with ab252435 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252435).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

      Immunohistochemical analysis of paraffin-embedded human lung tissue labeling C5a-R with ab252435 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on macrophages in human lung (PMID: 10027407) is observed. The section was incubated with ab252435 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252435).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling C5a-R with ab252435 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic and membranous staining on mainly neutrophils in human spleen (PMID: 22691190) is observed. The section was incubated with ab252435 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252435).

    • Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
      Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab272400? Please let us know so that we can cite the reference in this datasheet.

    ab272400 has not yet been referenced specifically in any publications.

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