Key features and details
- Mouse monoclonal [P12/1] to C5a-R
- Suitable for: Flow Cyt, WB, IHC-Fr, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-C5a-R antibody [P12/1]
See all C5a-R primary antibodies
DescriptionMouse monoclonal [P12/1] to C5a-R
No P12/1 signal after pre-incubation of HMC-1 cells with C5a, meaning that P12/1 does not displace C5a.
Tested applicationsSuitable for: Flow Cyt, WB, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Human
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab24036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/100.
Use 10µl of this working solution to label 5 x 105 cells in 100µl
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionReceptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsSulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
Cellular localizationCell membrane.
- Information by UniProt
- ANAPHYLATOXIN RECEPTOR antibody
- C5a anaphylatoxin chemotactic receptor antibody
- C5a anaphylatoxin receptor antibody
ab24036 has been referenced in 1 publication.
- Oppermann M et al. Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain. J Immunol 151:3785-94 (1993). PubMed: 8376805