Anti-C5a-R antibody [W17/1] (ab11884)

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Overview

  • Product name
    Anti-C5a-R antibody [W17/1]
    See all C5a-R primary antibodies
  • Description
    Mouse monoclonal [W17/1] to C5a-R
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, IHC-Frmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This information is considered to be commercially sensitive.

Properties

Applications

Our Abpromise guarantee covers the use of ab11884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Post-translational
      modifications
      Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • ANAPHYLATOXIN RECEPTOR antibody
        • C5a anaphylatoxin chemotactic receptor antibody
        • C5a anaphylatoxin receptor antibody
        • C5A antibody
        • C5a ligand antibody
        • C5a-R antibody
        • C5aR antibody
        • C5AR_HUMAN antibody
        • c5ar1 antibody
        • C5R1 antibody
        • CD88 antibody
        • CD88 antigen antibody
        • Complement component 5 receptor 1 antibody
        • Complement component 5 receptor 2 antibody
        • Complement component 5a receptor 1 antibody
        see all

      References

      This product has been referenced in:
      See all 2 Publications for this product

      Publishing research using ab11884? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Question

      Inquiry: I notice that in the latest batch of ab11884 the storage recommendations have changed from 4 degrees to 'aliquot and store at -20 degrees'. Is this due to some sort of change in the formulation or recent testing indicating better preservation of the antibody at this temperature? Thanks

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      Answer

      Thank you for contacting us.

      The formulation for this product has not changed. Recent tests showed thatab11884 can be stored at 4ºC as well as at -20ºC, this is why the storage information has been updated. In general the life timeof an antibody storedat -20ºC is about 5 years. As shown on the datasheet, there is no need to dilute the product but it is recommended to aliquot it and to avoid freeze / thaw cycles.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      What would be a suitable blocking peptide with this antibody?

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      Answer

      I have spoken to one of our scientists in the lab who advised me that to perform blocking experiments we always use the immunogen without its conjugation, therefore just the synthesized peptide should be suitable for your purpose. I was advised that we have had good results in peptide synthesis from a company called CSS, I hope this information will be useful. I also noticed that we did have access to the immunogen and have added the sequence clearly on the datasheet. I would like to also suggest you submit an Abreview with your results. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. We reward each Abreview with Abpoints which you can exchange for Amazon gift vouchers, conference attendance and many gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I wish you all the best with your research, please feel free to call me if you need further assistance,

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      Question

      I would like to know which region of C5aR does the antibody is raised against? As I'm looking for an anti-C5aR (CD88) antibody that doesn't not targets ligand binding site, i.e. intracellular region.

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      Answer

      The antibody was raised against the first extracellular domain of the protein termed "topo_dom" on the SwissProt database which you can access via our datasheet. I hope this information helps, please let me know if you need more details,

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