Overview

  • Product name

    Anti-C5b-9 + C5b-8 antibody [aE11]
  • Description

    Mouse monoclonal [aE11] to C5b-9 + C5b-8
  • Host species

    Mouse
  • Specificity

    The aE11 monoclonal was first reported to bind to a neoepitope exposed on polymerized C9 when incorporated into the human terminal complement complex (PubMed ID: 4035298). It was subsequently shown that this antibody also cross reacts with the C5b-8 complex and purified C8 alpha-gamma. It does not react with native C9 or C8 proteins (PubMed ID: 2424021).

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, ELISA, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Horse, Human, Pig, Monkey, Baboon
  • Immunogen

    Full length protein corresponding to Human C5b-9.

Properties

Applications

Our Abpromise guarantee covers the use of ab66768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 23803904

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 20688727
ELISA Use at an assay dependent concentration.
IHC-Fr 1/10.
  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • ab66768 staining C5b-9 + C5b-8 (red) in porcine liver tissue by Immunohistochemistry (Frozen sections).

      Tissue was taken from allotransplants of wild-type or a1,3-galactosyltransferase gene-knockout (GTKO) pig liver, 2 hours after grafting or at necroscopy (nec). Tissue was fixed with paraformaldehyde and blocked with 20% donkey serum for 1 hour at room temperature, before being incubated with primary antibody (1/100 in diluent).

    • ab66768 staining C5b-9 + C5b-8 in monkey retina cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

      Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton-X and blocked with 4% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. Counterstained with DAPI. Magnification: 20X.

      See Abreview

    • Immunohistochemical analysis of frozen tonsil tissue section labeling C5b-9 + C5b-8 with ab66768 at a dilution of 1/10.

      Vascular endothelia and dendritic cells of germinal center have strong and intensive label.

       

       

    References

    This product has been referenced in:

    • Artinger K  et al. The conundrum of postpartum thrombotic Microangiopathy: case report and considerations for management. BMC Nephrol 20:91 (2019). Read more (PubMed: 30871486) »
    • Zhu X  et al. All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-IL-1ß loop. Haematologica N/A:N/A (2019). Read more (PubMed: 30679324) »
    See all 17 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Monkey Tissue sections (Retina)
    Permeabilization
    Yes - 0.5 Triton-X
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 10 2014

    Question
    Answer

    Thank you for contacting us.

    The datasheet pdfs can be downloaded from Abcam website.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question
    Answer

    I am very pleased to hear you would like to accept our offer and test ab6678 in rabbit, ab8878 in IHC-P, and ab11862 in rabbit. These codes will give you 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for each antibody and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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