• Product name

    Anti-C5b-9 + C5b-8 antibody [aE11]
  • Description

    Mouse monoclonal [aE11] to C5b-9 + C5b-8
  • Host species

  • Specificity

    The aE11 monoclonal was first reported to bind to a neoepitope exposed on polymerized C9 when incorporated into the human terminal complement complex (PubMed ID: 4035298). It was subsequently shown that this antibody also cross reacts with the C5b-8 complex and purified C8 alpha-gamma. It does not react with native C9 or C8 proteins (PubMed ID: 2424021).

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, ELISA, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Horse, Human, Pig, Monkey, Baboon
  • Immunogen

    Full length protein corresponding to Human C5b-9.



Our Abpromise guarantee covers the use of ab66768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 23803904

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 20688727
ELISA Use at an assay dependent concentration.
IHC-Fr 1/10.
  • Application notes
    Is unsuitable for WB.
  • Target


    • ab66768 staining C5b-9 + C5b-8 (red) in porcine liver tissue by Immunohistochemistry (Frozen sections).

      Tissue was taken from allotransplants of wild-type or a1,3-galactosyltransferase gene-knockout (GTKO) pig liver, 2 hours after grafting or at necroscopy (nec). Tissue was fixed with paraformaldehyde and blocked with 20% donkey serum for 1 hour at room temperature, before being incubated with primary antibody (1/100 in diluent).

    • ab66768 staining C5b-9 + C5b-8 in monkey retina cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

      Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton-X and blocked with 4% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. Counterstained with DAPI. Magnification: 20X.

      See Abreview

    • Immunohistochemical analysis of frozen tonsil tissue section labeling C5b-9 + C5b-8 with ab66768 at a dilution of 1/10.

      Vascular endothelia and dendritic cells of germinal center have strong and intensive label.




    This product has been referenced in:

    • Artinger K  et al. The conundrum of postpartum thrombotic Microangiopathy: case report and considerations for management. BMC Nephrol 20:91 (2019). Read more (PubMed: 30871486) »
    • Zhu X  et al. All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-IL-1ß loop. Haematologica N/A:N/A (2019). Read more (PubMed: 30679324) »
    See all 17 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections)
    Monkey Tissue sections (Retina)
    Yes - 0.5 Triton-X
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jun 10 2014


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