• Product name
  • Description
    Rabbit polyclonal to C5b-9
  • Host species
  • Specificity
    This antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, IHC-FoFr, RID, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human C5b-9. Purified human SC5b-9 complex.



Our Abpromise guarantee covers the use of ab55811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
RID Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


  • Relevance
    Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells.
  • Cellular localization
  • Database links
  • Alternative names
    • C5 antibody
    • c5b 9 antibody
    • C6 antibody
    • C7 antibody
    • C8 antibody
    • C9 antibody
    • Complement component 5 antibody
    • Complement component 6 antibody
    • Complement component 7 antibody
    • Complement component 8 antibody
    • Complement component 9 antibody
    see all


  • ab55811 staining C5b-9 in human liver.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab55811 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55811, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Jeon H  et al. Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors. Sci Rep 8:5415 (2018). ELISA, ICC/IF . Read more (PubMed: 29615744) »
  • Tougan T  et al. Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5. Sci Rep 8:5052 (2018). Read more (PubMed: 29567995) »

See all 33 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Loading amount
80 µg
Gel Running Conditions
Non-reduced Denaturing
Mouse Tissue lysate - whole (Brain)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 23 2014

Thank you for contacting us.

The datasheet pdfs can be downloaded from Abcam website.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you.

Credit note ID: ####

The cred...

Read More

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

Read More

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IHC...

Read More

Thank you for your response and updates.

Wehope the second vial will work as it is expected, and please do letus know how you are getting on with this product.

If you need any further assistance, please do not hesitate to contact ou...

Read More

Thank you for your enquiry and your interest. My colleague has passed your enquiry to the Scientific Team.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investiga...

Read More

Thank you for contacting Abcam.

We have not tested ab55811 on rat tissue that does mean to say that it will not work, just that we have not tried it.

We are basing reactivity towards both human and mouse tissue based on in house tes...

Read More

Thank you for your enquiry. The reason that the molecular weight is different could be that the C5-b-9 complex degrades under proteolysis and therefore on a western blot, we are only seeing degradation products. In this case, it is the phosphoryla...

Read More

Thank you for your inquiry. I have contacted the originator of the ab55811 product and they have confirmed that this product was used in the following publication: Schafer, H., et al. 1986 (J. Immunol. 137, 1945), which suggests that this antibo...

Read More


Sign up