Anti-C5b-9 antibody (ab55811)

Rabbit polyclonal C5b-9 antibody. Validated in IHC, RID, ICC/IF and tested in Mouse, Human. Cited in 35 publication(s). Independently reviewed in 2 review(s).

Overview

  • Product name

  • Description

    Rabbit polyclonal to C5b-9
  • Host species

    Rabbit
  • Specificity

    This antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, IHC-FoFr, RID, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human C5b-9. Purified human SC5b-9 complex.

Properties

Applications

Our Abpromise guarantee covers the use of ab55811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
RID Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • C5 antibody
    • c5b 9 antibody
    • C6 antibody
    • C7 antibody
    • C8 antibody
    • C9 antibody
    • Complement component 5 antibody
    • Complement component 6 antibody
    • Complement component 7 antibody
    • Complement component 8 antibody
    • Complement component 9 antibody
    see all

Images

  • ab55811 staining C5b-9 in human liver.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab55811 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55811, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Fang L  et al. Myelin Oligodendrocyte Glycoprotein-IgG Contributes to Oligodendrocytopathy in the Presence of Complement, Distinct from Astrocytopathy Induced by AQP4-IgG. Neurosci Bull N/A:N/A (2019). Read more (PubMed: 31041694) »
  • Zhu X  et al. All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-IL-1ß loop. Haematologica N/A:N/A (2019). Read more (PubMed: 30679324) »
See all 41 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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1-2 of 2 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fibroblasts)
Permeabilization
Yes - NP40
Specification
Fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 18 2018

Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 23 2014

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