Recombinant Anti-NADK2 antibody [EPR13170] (ab181028)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13170] to NADK2
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NADK2 antibody [EPR13170]
See all NADK2 primary antibodies -
Description
Rabbit monoclonal [EPR13170] to NADK2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver and Human fetal brain lysates. HepG2 and Hela lysates. Human hepatocellular carcinoma and Human liver tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13170 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/2000. Predicted molecular weight: 49 kDa.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/2000. Predicted molecular weight: 49 kDa. |
Target
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Sequence similarities
Belongs to the UPF0465 family. - Information by UniProt
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Database links
- Entrez Gene: 133686 Human
- Entrez Gene: 365699 Rat
- SwissProt: Q4G0N4 Human
- SwissProt: Q8C5H8 Mouse
- SwissProt: Q1HCL7 Rat
- Unigene: 81907 Human
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Alternative names
- C5orf33 antibody
- CE033_HUMAN antibody
- Chromosome 5 open reading frame 33 antibody
see all
Images
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Immunohistochemical analysis of formalin fixed and paraffin embedded Human liver tissue labeling NADK2 with ab181028 at 1/100 dilution and counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of formalin fixed and paraffin embedded Human hepatocellular carcinoma tissue labeling NADK2 with ab181028 at 1/100 dilution and counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-NADK2 antibody [EPR13170] (ab181028) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal brain lysate
Lane 3 : HepG2 lysate
Lane 4 : Hela lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181028 has not yet been referenced specifically in any publications.