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    c5orf45-antibody-ab150917.pdf

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Anti-C5orf45 antibody (ab150917)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf45 antibody (ab150917)
  • Immunocytochemistry/ Immunofluorescence - Anti-C5orf45 antibody (ab150917)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf45 antibody (ab150917)

Key features and details

  • Rabbit polyclonal to C5orf45
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-C5orf45 antibody
  • Description

    Rabbit polyclonal to C5orf45
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 99-186 of Human C5orf45 (UniProt ID: Q6NTE8).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human Fallopian tube and endometrium tissue; ICC: A431 whole cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab150917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    C5orf45 belongs to the UPF0544 family.
  • Database links

    • Entrez Gene: 51149 Human
    • SwissProt: Q6NTE8 Human
    • Unigene: 741169 Human
    • Alternative names

      • Chromosome 5 open reading frame 45 antibody
      • DKFZp686L2452 antibody
      • LOC51149 antibody
      • MGC65027 antibody
      • MGC78537 antibody
      • UPF0544 protein C5orf45 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf45 antibody (ab150917)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf45 antibody (ab150917)

      Immunohistochemical analysis of human Fallopian tube tissue labeling C5orf45 in the nucleus of glandular cells with ab150917 at a 1/200 dilution.

      Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-C5orf45 antibody (ab150917)
      Immunocytochemistry/ Immunofluorescence - Anti-C5orf45 antibody (ab150917)

      Immunocytochemical analysis of A431 (Human epidermoid carcinoma cell line) whole cells labeling C5orf45 in the plasma membrane and cytoplasm with ab150917 at 2 µg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf45 antibody (ab150917)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf45 antibody (ab150917)

      Immunohistochemical analysis of human endometrium tissue labeling C5orf45 in the nucleus of glandular cells with ab150917 at a 1/200 dilution.

      Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab150917? Please let us know so that we can cite the reference in this datasheet.

    ab150917 has been referenced in 1 publication.

    • Staples CJ  et al. MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response. Cell Rep 16:2565-75 (2016). PubMed: 27568553

    Customer reviews and Q&As

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