Anti-C5orf45 antibody (ab150917)

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Overview

  • Product name

    Anti-C5orf45 antibody
    See all C5orf45 primary antibodies

  • Description

    Rabbit polyclonal to C5orf45

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment, corresponding to amino acids 99-186 of Human C5orf45 (UniProt ID: Q6NTE8).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human small intestine tissue; U 251 MG cell lysate, Human liver lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    C5orf45 belongs to the UPF0544 family.

  • Database links

    • Alternative names

      • Chromosome 5 open reading frame 45 antibody
      • DKFZp686L2452 antibody
      • LOC51149 antibody
      • MGC65027 antibody
      • MGC78537 antibody
      • UPF0544 protein C5orf45 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-C5orf45 antibody (ab150917)
      Immunocytochemistry/ Immunofluorescence - Anti-C5orf45 antibody (ab150917)
      Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab150917 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf45 antibody (ab150917)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf45 antibody (ab150917)
      Immunohistochemical analysis of Paraffin-embedded Human small intestine tissue labeling C5orf45 with ab150917 at 1/500 dilution.

    References

    This product has been referenced in:

    • Staples CJ  et al. MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response. Cell Rep 16:2565-75 (2016). Read more (PubMed: 27568553) »
    See 1 Publication for this product

    Publishing research using ab150917? Please let us know so that we can cite the reference in this datasheet.

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