Biotin Anti-C5a-R antibody [10/92] (ab54378)
Key features and details
- Biotin Rat monoclonal [10/92] to C5a-R
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Biotin Anti-C5a-R antibody [10/92]
See all C5a-R primary antibodies -
Description
Biotin Rat monoclonal [10/92] to C5a-R -
Host species
Rat -
Conjugation
Biotin -
Specificity
Clone 10/92 does not block the binding of the C5a to murine C5a-R. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse C5a-R. RBL-2H3 transfected cells expressing murine C5aR.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
10/92 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab54378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/50 - 1/100.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. |
Notes |
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Flow Cyt
1/50 - 1/100. Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. |
Target
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Function
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsSulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12273 Mouse
- SwissProt: P30993 Mouse
- SwissProt: Q3TZ86 Mouse
- Unigene: 247623 Mouse
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Alternative names
- ANAPHYLATOXIN RECEPTOR antibody
- C5a anaphylatoxin chemotactic receptor antibody
- C5a anaphylatoxin receptor antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab54378 has been referenced in 1 publication.
- Copland DA et al. Systemic and local anti-C5 therapy reduces the disease severity in experimental autoimmune uveoretinitis. Clin Exp Immunol 159:303-14 (2010). IHC-Fr ; Mouse . PubMed: 20002447