Anti-C6ORF10 antibody (ab222839)
Key features and details
- Rabbit polyclonal to C6ORF10
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-C6ORF10 antibody -
Description
Rabbit polyclonal to C6ORF10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human C6ORF10 aa 200-500.
Database link: Q5SRN2 -
Positive control
- WB: Mouse kidney tissue lysate. IHC-P: Human liver and spleen tissues. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/2000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10665 Human
- SwissProt: Q5SRN2 Human
- Unigene: 567414 Human
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Alternative names
- C6orf10 antibody
- CF010_HUMAN antibody
- Chromosome 6 open reading frame 10 antibody
see all
Images
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for C6ORF10 (green) using ab222839 at 1/100 dilution in ICC/IF. Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as secondary antibody.
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Paraffin-embedded human liver tissue stained for C6ORF10 using ab222839 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human spleen tissue stained for C6ORF10 using ab222839 at 1/100 dilution in immunohistochemical analysis.
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Anti-C6ORF10 antibody (ab222839) at 1/2000 dilution + mouse kidney tissue lysate
Secondary
Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab222839 has not yet been referenced specifically in any publications.