Overview

  • Product name

    Anti-C6ORF10 antibody
  • Description

    Rabbit polyclonal to C6ORF10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human C6ORF10 aa 239-476.
    Sequence:

    TARSQNSREENKEALKNDIIFTNSVESLKSAHIKEPEREGKGTDLEKDKI GMEVKVDSDAGIPKRQETQLKISEMSIPQGQGAQIKKSVSDVPRGQESQV KKSESGVPKGQEAQVTKSGLVVLKGQEAQVEKSEMGVPRRQESQVKKSQS GVSKGQEAQVKKRESVVLKGQEAQVEKSELKVPKGQEGQVEKTEADVPKE QEVQEKKSEAGVLKGPESQVKNTEVSVPETLESQVKKS


    Database link: Q5SRN2

  • Positive control

    • WB: Mouse kidney tissue lysate. IHC-P: Human liver and spleen tissues. ICC/IF: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.

Target

Images

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for C6ORF10 (green) using ab222839 at 1/100 dilution in ICC/IF. Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as secondary antibody.

  • Paraffin-embedded human liver tissue stained for C6ORF10 using ab222839 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human spleen tissue stained for C6ORF10 using ab222839 at 1/100 dilution in immunohistochemical analysis.

  • Anti-C6ORF10 antibody (ab222839) at 1/2000 dilution + mouse kidney tissue lysate

    Secondary
    Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

References

ab222839 has not yet been referenced specifically in any publications.

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