Overview

  • Product name

    Anti-C6orf130 antibody
  • Description

    Rabbit polyclonal to C6orf130
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to N terminal amino acids 1-78 (MASSLNEDPE ASRITYVKGD LFACPKTDSL AHCISEDCRM GAGIAVLFKK KFGGVQELLN QQKKSGEVAV LKRDGRYI) of Human C6orf130.

  • Positive control

    • Human small intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and nucleoli. Recommended concentration of ab122032 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122032 at 1/200 dilution staining C6orf130 in paraffin-embedded Human small intestine by Immunohistochemistry.

References

ab122032 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoblastoid)
Loading amount
30 µg
Specification
lymphoblastoid
Gel Running Conditions
Reduced Denaturing (12.5)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 12 2012

Answer

DISCOUNT CODE: 0000

Expiration date: 13. November 2012
I am very pleased to hear your customer would like to accept our offer and test ab122032 in WB.
This code will give your customer 1 free primary antibody before the expiration date.
To redeem this offer, please submit an Abreview for WB and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.
The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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