Product nameAnti-C6orf134 antibody
See all C6orf134 primary antibodies
DescriptionRabbit polyclonal to C6orf134
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
- HepG2 Cell Lysate IF/ICC: U-87 MG cell line.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab58742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1:1562500.
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionSpecifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development.
Sequence similaritiesBelongs to the acetyltransferase ATAT1 family.
- Information by UniProt
- Acetyltransferase mec 17 homolog antibody
- Acetyltransferase mec-17 homolog antibody
- Alpha TAT antibody
ICC/IF image of ab58742 stained U-87 MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58742, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-C6orf134 antibody (ab58742) at 2.5 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Gel concentration: 12%
This product has been referenced in:
- Xiaojun W et al. Acetylated a-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline(Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica. Sci Rep 6:32257 (2016). WB ; Rat . Read more (PubMed: 27577858) »
- Gu S et al. Loss of a-Tubulin Acetylation Is Associated with TGF-ß-induced Epithelial-Mesenchymal Transition. J Biol Chem 291:5396-405 (2016). Read more (PubMed: 26763233) »