Key features and details
- Rabbit polyclonal to C6orf134/TAT
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C6orf134/TAT antibody
See all C6orf134/TAT primary antibodies
DescriptionRabbit polyclonal to C6orf134/TAT
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
- WB: HepG2 cell lysate; Human ovary tumor lysate.
This product was previously labelled as C6orf134
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab58742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 - 3 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionSpecifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development.
Sequence similaritiesBelongs to the acetyltransferase ATAT1 family.
- Information by UniProt
- Acetyltransferase mec 17 homolog antibody
- Acetyltransferase mec-17 homolog antibody
- Alpha TAT antibody
All lanes : Anti-C6orf134/TAT antibody (ab58742) at 3 µg/ml
Lane 1 : Human ovary tumor lysate
Lane 2 : Human lung tumor lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 36 kDa
Western blot analysis labeling C6orf134/TAT with ab58742.
Anti-C6orf134/TAT antibody (ab58742) at 2.5 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Gel concentration: 12%
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab58742 has been referenced in 10 publications.
- Zeng QZ et al. Paclitaxel Enhances the Innate Immunity by Promoting NLRP3 Inflammasome Activation in Macrophages. Front Immunol 10:72 (2019). PubMed: 30761140
- Shifeng L et al. Ac-SDKP increases a-TAT 1 and promotes the apoptosis in lung fibroblasts and epithelial cells double-stimulated with TGF-ß1 and silica. Toxicol Appl Pharmacol 369:17-29 (2019). PubMed: 30826375
- Li CG et al. Evodiamine Augments NLRP3 Inflammasome Activation and Anti-bacterial Responses Through Inducing a-Tubulin Acetylation. Front Pharmacol 10:290 (2019). PubMed: 30971927
- Li Z et al. hnRNPK modulates selective quality-control autophagy by downregulating the expression of HDAC6 in 293 cells. Int J Oncol 53:2200-2212 (2018). PubMed: 30106132
- Lee CC et al. Alpha-tubulin acetyltransferase/MEC-17 regulates cancer cell migration and invasion through epithelial-mesenchymal transition suppression and cell polarity disruption. Sci Rep 8:17477 (2018). PubMed: 30504808
- Gu S et al. Loss of a-Tubulin Acetylation Is Associated with TGF-ß-induced Epithelial-Mesenchymal Transition. J Biol Chem 291:5396-405 (2016). PubMed: 26763233
- Xiaojun W et al. Acetylated a-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline(Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica. Sci Rep 6:32257 (2016). WB ; Rat . PubMed: 27577858
- Chauhan S et al. Pharmaceutical screen identifies novel target processes for activation of autophagy with a broad translational potential. Nat Commun 6:8620 (2015). PubMed: 26503418
- Wang B et al. Microtubule acetylation amplifies p38 kinase signalling and anti-inflammatory IL-10 production. Nat Commun 5:3479 (2014). PubMed: 24632940
- Kalebic N et al. Tubulin acetyltransferase aTAT1 destabilizes microtubules independently of its acetylation activity. Mol Cell Biol 33:1114-23 (2013). PubMed: 23275437