Key features and details
- Rabbit polyclonal to C6orf15
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C6orf15 antibody
DescriptionRabbit polyclonal to C6orf15
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- Human cerebellum tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
Tissue specificityExpressed in skin and tonsils.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- C6orf15 antibody
- CF015_HUMAN antibody
- Protein STG antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab121137 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical staining of paraffin embedded human cerebellum tissue stained with anti-C60rf15 antibody (ab121137; dilution 1:150) showing strong nuclear positivity in purkinje cells and cells of molecular layer.
ab121137 has not yet been referenced specifically in any publications.