Product nameAnti-C6orf174 antibody
DescriptionRabbit polyclonal to C6orf174
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human Plasma; Human testis tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||1/250 - 1/500.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
- Information by UniProt
- C6orf174 antibody
- CF174_HUMAN antibody
- Uncharacterized protein C6orf174 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab122272 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-C6orf174 antibody (ab122272) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U251-MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab122272, at 1/30 dilution, staining C6orf174 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
ab122272 has not yet been referenced specifically in any publications.