Key features and details
- Rabbit polyclonal to C6ORF182
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C6ORF182 antibody
See all C6ORF182 primary antibodies
DescriptionRabbit polyclonal to C6ORF182
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: CEM, HEK-293T, HeLa, Jurkat and A549 whole cell lysates. IHC-P: Human tonsil and skin tissues.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 46,54 kDa.|
RelevanceThe exact functions of C6orf182 remain unknown but it is thought to be a centrosomal protein which may be required for microtubule attachment to centrosomes.
Cellular localizationCytoplasmic; cytoskeleton, microtubule. Centrosome.
- BA487F23.2 antibody
- Centrosomal protein of 57 kDa-related protein antibody
- Cep57-related protein antibody
Paraffin-embedded human skin tissue stained for CEP57L1 using ab229864 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human tonsil tissue stained for CEP57L1 using ab229864 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-C6ORF182 antibody (ab229864) at 1/1000 dilution
Lane 1 : CEM whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 46,54 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229864 has not yet been referenced specifically in any publications.