Key features and details
- Rabbit polyclonal to C6orf57
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C6orf57 antibody
DescriptionRabbit polyclonal to C6orf57
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- IHC: Human testis and fallopian tube. WB: C6orf57 over-expression HEK293T lysate. ICC/IF: Human cell line A-431
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesBelongs to the UPF0369 family.
- Information by UniProt
- C6orf57 antibody
- CF057_HUMAN antibody
- Chromosome 6 open reading frame 57 antibody
- UPF0369 protein C6orf57 antibody
ab122196 at 1/25 dilution staining C6orf57 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
ab122196 at 1/25 dilution staining C6orf57 in Paraffin-embedded Human fallopian tube tissue by Immunohistochemistry.
Immunocytochemistry/immunofluorescence analysis of human cell line A-431 labelling C6orf57 with ab122196 at 2 µg/mL.
ab122196 at 1/25 dilution staining C6orf57 in Paraffin-embedded Human Duodenum tissue by Immunohistochemistry.
All lanes : Anti-C6orf57 antibody (ab122196) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : C6orf57-transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
ab122196 has not yet been referenced specifically in any publications.