Key features and details
- Rabbit polyclonal to C6orf72
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C6orf72 antibody
DescriptionRabbit polyclonal to C6orf72
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human gall bladder
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59.9% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
- Information by UniProt
- C6orf72 antibody
- CF072_HUMAN antibody
- dJ12G14.2 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and nucleus but not nucleoli. Recommended concentration of ab121528 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121528, at 1/50 - 1/200 dilution, staining C6orf72 in paraffin-embedded Human gall bladder by Immunohistochemistry.
All lanes : Anti-C6orf72 antibody (ab121528)
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4 lysate
Lane 3 : U-251 MG lysate
Lane 4 : Human Plasma
Lane 5 : Liver lysate
Lane 6 : Tonsil lysate
ab121528 has not yet been referenced specifically in any publications.