Overview

  • Product name
  • Description
    Rabbit polyclonal to C7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human C7 aa 381-411 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P10643

  • Positive control
    • Ramos cell lysate; Human lymph tissue; Ramos cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176004 was purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 93 kDa.

Target

  • Function
    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.
  • Involvement in disease
    Defects in C7 are a cause of complement component 7 deficiency (C7D) [MIM:610102]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • Sequence similarities
    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 2 Sushi (CCP/SCR) domains.
    Contains 2 TSP type-1 domains.
  • Post-translational
    modifications
    C7 has 28 disulfide bridges.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C7 antibody
    • CO7_HUMAN antibody
    • complement component 7 antibody
    • Complement component C7 antibody

Images

  • Flow cytometric analysis of Ramos cells labeling C7 with ab176004 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed,paraffin-embedded Human lymph tissue labeling C7 with ab176004 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-C7 antibody (ab176004) at 1/1000 dilution + Ramos cell lysate at 35 µg

    Predicted band size: 93 kDa

References

ab176004 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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