Recombinant Anti-C7orf42 antibody [EPR15381] (ab185948)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15381] to C7orf42
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-C7orf42 antibody [EPR15381]
See all C7orf42 primary antibodies -
Description
Rabbit monoclonal [EPR15381] to C7orf42 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Hela, MCF-7, A549, HepG2, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates. Hela cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15381 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/210.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
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Notes |
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Flow Cyt (Intra)
1/210. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
Target
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Sequence similarities
Belongs to the UPF0458 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 55069 Human
- Entrez Gene: 71667 Mouse
- Entrez Gene: 288616 Rat
- SwissProt: Q9NWD8 Human
- SwissProt: Q3TBN1 Mouse
- SwissProt: Q6AY76 Rat
- Unigene: 488478 Human
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Alternative names
- C7orf42 antibody
- CG042_HUMAN antibody
- Chromosome 7 open reading frame 42 antibody
see all
Images
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All lanes : Anti-C7orf42 antibody [EPR15381] (ab185948) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDaBlocking buffer: 5% NFDM/TBST
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Intracellular Flow Cytometry analysis of HeLa cells usingab185948 at 1/210 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
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All lanes : Anti-C7orf42 antibody [EPR15381] (ab185948) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDaBlocking buffer: 5% NFDM/TBST
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab185948 has not yet been referenced specifically in any publications.