Key features and details
- Mouse monoclonal [056B-373] to C8
- Suitable for: WB, ELISA, IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-C8 antibody [056B-373]
DescriptionMouse monoclonal [056B-373] to C8
Tested applicationsSuitable for: WB, ELISA, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Human
Purified human C8
- Kidney from post streptoccal glomerulonephritis patients.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab59140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 151 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceC8 is synthesised in the liver, monocytes and fibroblasts and is a plasma protein consisting of three non identical polypeptide chains. The alpha-chain (64kDa) and gamma chain (22kDa) are disulphide linked, these associate non-covalently with the beta-chain (64kDa), to form the native C8 molecule with a molecular weight of 151kDa. Normal plasma concentration is 80mg/L. C8 is one of the terminal proteins of the complement pathways. C8 binds to the membrane bound C5b-7, C5b-8 combines with C9 to form the membrane attack complex (MAC). The MAC forms a hydrophilic, protein walled, transmembrane channel, formation of which leads to cell lysis and destruction.
- C8A antibody
- C8B antibody
- C8G antibody
ab59140 has not yet been referenced specifically in any publications.