Recombinant Anti-C8G antibody [EPR11274] - BSA and Azide free (ab250407)
![Western blot - Anti-C8G antibody [EPR11274] - BSA and Azide free (ab250407)](https://www.abcam.com/ps/products/250/ab250407/Images/ab250407-402721-anti-c8g-antibody-epr11274-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-C8G antibody [EPR11274] - BSA and Azide free (ab250407)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11274] to C8G - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-C8G antibody [EPR11274] - BSA and Azide free
See all C8G primary antibodies -
Description
Rabbit monoclonal [EPR11274] to C8G - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250407 is the carrier-free version of ab181182.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11274 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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| Notes |
|---|
|
ICC
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Function
C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 733 Human
- Entrez Gene: 69379 Mouse
- Entrez Gene: 296545 Rat
- Omim: 120930 Human
- SwissProt: P07360 Human
- SwissProt: Q8VCG4 Mouse
- Unigene: 1285 Human
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Alternative names
- C8C antibody
- C8G antibody
- CO8G_HUMAN antibody
see all
Images
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Western blot - Anti-C8G antibody [EPR11274] - BSA and Azide free (ab250407)All lanes : Anti-C8G antibody [EPR11274] - C-terminal (ab181182) at 1/1000 dilution
Lane 1 : Human colon cancer lysate
Lane 2 : Human plasma
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDaThis data was developed using ab181182, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-C8G antibody [EPR11274] - BSA and Azide free (ab250407)](https://www.abcam.com/ps/products/250/ab250407/Images/ab250407-1-anti-c8g-antibody-epr11274-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-C8G antibody [EPR11274] - BSA and Azide free (ab250407)This data was developed using ab181182, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Human SW480 cells, staining C8G (red) with ab181182 at 1/100 dilution. An Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). -
![Anti-C8G antibody [EPR11274] - BSA and Azide free (ab250407)](https://www.abcam.com/ps/products/250/ab250407/Images/ab250407-3-benefits-of-recombinant-antibodies.png)
Anti-C8G antibody [EPR11274] - BSA and Azide free (ab250407)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250407 has not yet been referenced specifically in any publications.
