RecombinantRabMAb

Recombinant Anti-C8G antibody [EPR11274] - C-terminal (ab181182)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11274] to C8G - C-terminal
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-C8G antibody [EPR11274] - C-terminal
    See all C8G primary antibodies

  • Description

    Rabbit monoclonal [EPR11274] to C8G - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C8G aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P07360

  • Positive control

    • Human colon cancer lysate and plasma. SW480 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11274

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC/IF 1/50 - 1/100.

Target

Images

  • Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
    Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
    All lanes : Anti-C8G antibody [EPR11274] - C-terminal (ab181182) at 1/1000 dilution

    Lane 1 : Human colon cancer lysate
    Lane 2 : Human plasma

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
    Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)

    Immunofluorescence analysis of Human SW480 cells, staining C8G (red) with ab181182 at 1/100 dilution. An Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

References (0)

Publishing research using ab181182? Please let us know so that we can cite the reference in this datasheet.

ab181182 has not yet been referenced specifically in any publications.

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