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  1. Link

    c8g-antibody-epr11274-c-terminal-ab181182.pdf

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Immunology Innate Immunity Complement Alternative Pathway
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RecombinantRabMAb

Recombinant Anti-C8G antibody [EPR11274] - C-terminal (ab181182)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
  • Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
  • Anti-C8G antibody [EPR11274] - C-terminal (ab181182)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11274] to C8G - C-terminal
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-C8G antibody [EPR11274] - C-terminal
    See all C8G primary antibodies
  • Description

    Rabbit monoclonal [EPR11274] to C8G - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human colon cancer lysate and plasma. SW480 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11274
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Alternative Pathway

Associated products

  • Alternative Versions

    • Anti-C8G antibody [EPR11274] - BSA and Azide free (ab250407)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human C8G protein (ab113121)
  • Related Products

    • Recombinant Human C8G protein (ab113121)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC/IF
1/50 - 1/100.

Target

  • Function

    C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P07360 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 733 Human
    • Entrez Gene: 69379 Mouse
    • Entrez Gene: 296545 Rat
    • Omim: 120930 Human
    • SwissProt: P07360 Human
    • SwissProt: Q8VCG4 Mouse
    • Unigene: 1285 Human
    • Alternative names

      • C8C antibody
      • C8G antibody
      • CO8G_HUMAN antibody
      • Complement component 8 gamma polypeptide antibody
      • Complement component C8 gamma chain antibody
      • MGC142186 antibody
      see all

    Images

    • Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
      Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
      All lanes : Anti-C8G antibody [EPR11274] - C-terminal (ab181182) at 1/1000 dilution

      Lane 1 : Human colon cancer lysate
      Lane 2 : Human plasma

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
      Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)

      Immunofluorescence analysis of Human SW480 cells, staining C8G (red) with ab181182 at 1/100 dilution. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

    • Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
      Anti-C8G antibody [EPR11274] - C-terminal (ab181182)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab181182? Please let us know so that we can cite the reference in this datasheet.

    ab181182 has not yet been referenced specifically in any publications.

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