Recombinant
RabMAb

Recombinant Anti-C8G antibody [EPR11274] - C-terminal (ab181182)

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Overview

  • Product name

    Anti-C8G antibody [EPR11274] - C-terminal
    See all C8G primary antibodies

  • Description

    Rabbit monoclonal [EPR11274] to C8G - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C8G aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P07360

  • Positive control

    • Human colon cancer lysate and plasma. SW480 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11274

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC/IF 1/50 - 1/100.

Target

Images

  • Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
    Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
    All lanes : Anti-C8G antibody [EPR11274] - C-terminal (ab181182) at 1/1000 dilution

    Lane 1 : Human colon cancer lysate
    Lane 2 : Human plasma

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
    Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)

    Immunofluorescence analysis of Human SW480 cells, staining C8G (red) with ab181182 at 1/100 dilution. An Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

References

ab181182 has not yet been referenced specifically in any publications.

Publishing research using ab181182? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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