Anti-C8orf55 antibody (ab121743)
Key features and details
- Rabbit polyclonal to C8orf55
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C8orf55 antibody -
Description
Rabbit polyclonal to C8orf55 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 123-207 (LLGWDDRAFY LEARFVSLRD GFVCALLRFR QHLLGTSPER VVQHLCQRRV EPPELPADLQ HWISYNEASS QLLRMESGLS DVTKDQ) of Human C8orf55.
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Positive control
- IHC: Human cerebellum. kidney, small intestine and Fallopian tube. WB: RT-4 and U-251 MG cell lysate; Human Liver lysate. ICC/IF: Human cell line U-251MG
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 0.04 µg/ml.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC
Use a concentration of 0.04 µg/ml. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Sequence similarities
Belongs to the UPF0670 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 51337 Human
- SwissProt: Q8WUY1 Human
- Unigene: 368402 Human
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Alternative names
- C8orf55 antibody
- CH055_HUMAN antibody
- DSCD75 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121743 is 0.04 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-C8orf55 antibody (ab121743) at 0.04 µg/ml
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 :
Human tonsil lysate
Developed using the ECL technique. -
ab121743, at a 1/1000 dilution staining C8orf55 in paraffin-embedded Human small intestine tissue by Immunohistochemistry.
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ab121743, at a 1/1000 dilution staining C8orf55 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
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ab121743, at a 1/1000 dilution staining C8orf55 in paraffin-embedded Human fallopian tube tissue by Immunohistochemistry.
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ab121743, at a 1/1000 dilution staining C8orf55 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab121743 has been referenced in 1 publication.
- Blomme A et al. THEM6-mediated reprogramming of lipid metabolism supports treatment resistance in prostate cancer. EMBO Mol Med 14:e14764 (2022). PubMed: 35014179