Anti-C8orf55 antibody (ab121743)
Key features and details
- Rabbit polyclonal to C8orf55
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-C8orf55 antibody -
Description
Rabbit polyclonal to C8orf55 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanWB Human
-
Immunogen
antigen sequence, corresponding to amino acids 123-207 (LLGWDDRAFY LEARFVSLRD GFVCALLRFR QHLLGTSPER VVQHLCQRRV EPPELPADLQ HWISYNEASS QLLRMESGLS DVTKDQ) of Human C8orf55.
-
Positive control
- IHC: Human cerebellum. kidney, small intestine and Fallopian tube. WB: RT-4 and U-251 MG cell lysate; Human Liver lysate. ICC/IF: Human cell line U-251MG
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| Notes |
|---|
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
-
Sequence similarities
Belongs to the UPF0670 family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 51337 Human
- SwissProt: Q8WUY1 Human
- Unigene: 368402 Human
-
Alternative names
- C8orf55 antibody
- CH055_HUMAN antibody
- DSCD75 antibody
see all
Images
-
Immunocytochemistry - Anti-C8orf55 antibody (ab121743)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121743 is 0.04 µg/ml. Cells treated with PFA/Triton X-100.
-
Western blot - Anti-C8orf55 antibody (ab121743)All lanes : Anti-C8orf55 antibody (ab121743) at 0.04 µg/ml
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 :
Human tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf55 antibody (ab121743)ab121743, at a 1/1000 dilution staining C8orf55 in paraffin-embedded Human small intestine tissue by Immunohistochemistry.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf55 antibody (ab121743)ab121743, at a 1/1000 dilution staining C8orf55 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf55 antibody (ab121743)ab121743, at a 1/1000 dilution staining C8orf55 in paraffin-embedded Human fallopian tube tissue by Immunohistochemistry.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf55 antibody (ab121743)ab121743, at a 1/1000 dilution staining C8orf55 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
Protocols
Datasheets and documents
References (0)
ab121743 has not yet been referenced specifically in any publications.
