Overview

  • Product name
    Anti-C8orf55 antibody
    See all C8orf55 primary antibodies
  • Description
    Rabbit polyclonal to C8orf55
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 123-207 (LLGWDDRAFY LEARFVSLRD GFVCALLRFR QHLLGTSPER VVQHLCQRRV EPPELPADLQ HWISYNEASS QLLRMESGLS DVTKDQ) of Human C8orf55.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human cerebellum tissue;
      RT-4 and U-251 MG cell lysate; Human Liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities
    Belongs to the UPF0670 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • C8orf55 antibody
      • CH055_HUMAN antibody
      • DSCD75 antibody
      • Mesenchymal stem cell protein DSCD75 antibody
      • mesenchymal stem cell protein DSCD75 homolog antibody
      • THEM6 antibody
      • Thioesterase superfamily member 6 antibody
      • UPF0670 protein C8orf55 antibody
      • UPF0670 protein C8orf55 homolog antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-C8orf55 antibody (ab121743)
      Immunocytochemistry/ Immunofluorescence - Anti-C8orf55 antibody (ab121743)
      Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121743 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf55 antibody (ab121743)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf55 antibody (ab121743)
      ab121743, at a 1/1000 dilution staining C8orf55 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
    • Western blot - Anti-C8orf55 antibody (ab121743)
      Western blot - Anti-C8orf55 antibody (ab121743)
      All lanes : Anti-C8orf55 antibody (ab121743) at 1/250 dilution

      Lane 1 : RT-4 lysate

      Lane 2 : U-251 MG lysate

      Lane 3 : Human plasma

      Lane 4 : Human liver lysate

      Lane 5 :
      Human tonsil lysate

      Developed using the ECL technique.

    References

    ab121743 has not yet been referenced specifically in any publications.

    Publishing research using ab121743? Please let us know so that we can cite the reference in this datasheet.

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