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    c9-antibody-53-ab17931.pdf

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Immunology Innate Immunity Complement MAC
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Anti-C9 antibody [53] (ab17931)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [53] (ab17931)
  • Flow Cytometry - Anti-C9 antibody [53] (ab17931)

Key features and details

  • Mouse monoclonal [53] to C9
  • Suitable for: ELISA, WB, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-C9 antibody [53]
    See all C9 primary antibodies
  • Description

    Mouse monoclonal [53] to C9
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified, full length native human complement factor C9 protein.

  • General notes

    ab17931 recognizes C9 in human serum diluted 1:50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 µg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 0.754% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Protein A/G purified.
  • Primary antibody notes

    ab17931 recognizes C9 in human serum diluted 1:50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 µg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate.
  • Clonality

    Monoclonal
  • Clone number

    53
  • Myeloma

    Sp2
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • MAC

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human C9 protein (ab114199)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa. PubMed: 20707004
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P
Use a concentration of 2 µg/ml.
Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa. PubMed: 20707004
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P
Use a concentration of 2 µg/ml.

Target

  • Function

    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • Sequence similarities

    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 1 TSP type-1 domain.
  • Post-translational
    modifications

    Thrombin cleaves factor C9 to produce C9a and C9b.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
  • Target information above from: UniProt accession P02748 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 735 Human
    • Omim: 120940 Human
    • SwissProt: P02748 Human
    • Unigene: 654443 Human
    • Alternative names

      • ARMD15 antibody
      • C9 antibody
      • C9 deficiency antibody
      • C9 deficiency with dermatomyositis antibody
      • C9D antibody
      • CO9_HUMAN antibody
      • Complement component 9 antibody
      • Complement component 9 deficiency antibody
      • Complement component C9 antibody
      • Complement component C9b antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [53] (ab17931)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [53] (ab17931)
      Ab17931 staining human normal liver. Staining is localized to the cytoplasm.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Flow Cytometry - Anti-C9 antibody [53] (ab17931)
      Flow Cytometry - Anti-C9 antibody [53] (ab17931)
      Overlay histogram showing HeLa cells stained with ab17931 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17931, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab17931? Please let us know so that we can cite the reference in this datasheet.

    ab17931 has been referenced in 3 publications.

    • Xu X  et al. Silencing of NONO inhibits abdominal aortic aneurysm in apolipoprotein E-knockout mice via collagen deposition and inflammatory inhibition. J Cell Mol Med 23:7449-7461 (2019). PubMed: 31512366
    • Kelly KJ  et al. Renal C3 complement component: feed forward to diabetic kidney disease. Am J Nephrol 41:48-56 (2015). PubMed: 25662584
    • Chong PK  et al. Upregulation of plasma C9 protein in gastric cancer patients. Proteomics 10:3210-21 (2010). WB, IHC-P ; Human . PubMed: 20707004

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