Key features and details
- Guinea pig polyclonal to C9
- Suitable for: IHC-P, WB
- Reacts with: Rabbit
- Isotype: IgG
Product nameAnti-C9 antibody
See all C9 primary antibodies
DescriptionGuinea pig polyclonal to C9
Host speciesGuinea pig
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rabbit
Recombinant fragment (His-T7-tag) corresponding to Rabbit C9 aa 139-519. (Expressed in E.coli).
PRLTCRNREVQESELARTAGYGINILGMDPLATPFDNEYYHGLCDRVWDG NTLTHYRKPWNVAVLAYETKIDKNFRTEYYEEQMQAFKSIIEEETSNFNA NLALKFTPTEAKASKAEEASPKNKSLDDNDKGFSSKFQFSYSKNETYQLF LSYSSQKEKMFLLVKGIIQLGRFVMKNRGVMLTNTFLDDIKSLPTTYEKG EYFAFLETYGTHYSSSGSLGGRYELIYVLDKASMKEKGIELNDIKKCLGF DLDLSLNIPGKSAGLSLTGQANKNNCLKSGHGNAVNITRANLIDDVISLI RGGTQKFAFELKEKLLTKAKMVDVTDFINWASSLSDAPVLINQKLSPIYN LIPVKIKDAHQKRQNLERGIEDYINEFSTKK
Database link: P48747
- IHC-P: Rabbit liver tissue. WB: Rabbit kidney and heart lysates; Recombinant rabbit C9 protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232687 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 63 kDa.|
FunctionConstituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
Involvement in diseaseDefects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
Sequence similaritiesBelongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 1 TSP type-1 domain.
modificationsThrombin cleaves factor C9 to produce C9a and C9b.
Phosphorylation sites are present in the extracelllular medium.
Cellular localizationSecreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
- Information by UniProt
- ARMD15 antibody
- C9 antibody
- C9 deficiency antibody
Paraffin-embedded rabbit liver tissue stained for C9 using ab232687 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-C9 antibody (ab232687) at 2 µg/ml + Recombinant rabbit C9 protein
Predicted band size: 63 kDa
All lanes : Anti-C9 antibody (ab232687) at 2 µg/ml
Lane 1 : Rabbit kidney lysate
Lane 2 : Rabbit heart lysate
Predicted band size: 63 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232687 has not yet been referenced specifically in any publications.