Overview

  • Product name

  • Description

    Rabbit polyclonal to C9
  • Host species

    Rabbit
  • Specificity

    This product may also detect C9 within the C5b-9 complex, but we have no evidence to show this. Any feedback from our customers is welcome. We recommend ab55811 for specifically detecting the C5b-9 complex.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human C9.

    Read Abcam's proprietary immunogen policy (Peptide available as ab71328.)

  • Positive control

    • Recombinant Human C9 protein (ab114199) can be used as a positive control in WB. This antibody gave a positive signal in the following tissue lysates: Kidney (Human) Placenta (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab64011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).

Target

  • Function

    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • Sequence similarities

    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 1 TSP type-1 domain.
  • Post-translational
    modifications

    Thrombin cleaves factor C9 to produce C9a and C9b.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARMD15 antibody
    • C9 antibody
    • C9 deficiency antibody
    • C9 deficiency with dermatomyositis antibody
    • C9D antibody
    • CO9_HUMAN antibody
    • Complement component 9 antibody
    • Complement component 9 deficiency antibody
    • Complement component C9 antibody
    • Complement component C9b antibody
    see all

Images

  • All lanes : Anti-C9 antibody (ab64011) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 40,70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 minutes


    We hypothesize that the 70 kDa band represents the glycosylated C9 protein (predicted molecular weight 63), whereas the 40 kDa band represents the glycosylated C9b cleavage product (predicted molecular weight 33 kDa).

References

This product has been referenced in:

  • Papareddy P  et al. C-terminal Peptides of Tissue Factor Pathway Inhibitor Are Novel Host Defense Molecules. J Biol Chem 285:28387-98 (2010). WB . Read more (PubMed: 20592020) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your inquiry.

I can confirm that both antibodies may also detect C9 within the C5b-9 complex, but we have no evidence to show this. Any feedback from our customers is welcome.

Both antibodieswere raised against an immunogen from C9 (a component of the C5b-9 complex).

We recommend ab55811 for specifically detecting the C5b-9 complex.

https://www.abcam.com/index.html?datasheet=55811 (or use the following: https://www.abcam.com/index.html?datasheet=55811).

I hope this information is helpful and wish you a good weekend.

Read More

Answer

Thank you for your enquiry. The reason that the molecular weight is different could be that the C5-b-9 complex degrades under proteolysis and therefore on a western blot, we are only seeing degradation products. In this case, it is the phosphorylated C9 which we are seeing. An example can be seen in the WB image for ab83076. "C5 is cleaved into C5 alpha and C5 beta chains, joined by a disulphide bond. C5 alpha is cleaved leaving C5a (anaphylatoxin, which is released) and C5b (alpha’ + beta chains). C5b then binds sequentially to C6, C7, C8 and C9 to form the Membrane attach complex (MAC), also known as C5b-9. In rat urine, C5b-9 has been shown to be degraded presumably by proteolysis (Kerjaschkl et al., 1989). We hypothesise that the bands at 30- and 55-kDa correspond to degradation products." I hope this information will be helpful.  

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