Overview

  • Product name
  • Description
    Rabbit polyclonal to C9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human C9.

    (Peptide available as ab94838.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human placenta; Human plasma total protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 63 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
    • Tissue specificity
      Plasma.
    • Involvement in disease
      Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    • Sequence similarities
      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 EGF-like domain.
      Contains 1 LDL-receptor class A domain.
      Contains 1 MACPF domain.
      Contains 1 TSP type-1 domain.
    • Post-translational
      modifications
      Thrombin cleaves factor C9 to produce C9a and C9b.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization
      Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARMD15 antibody
      • C9 antibody
      • C9 deficiency antibody
      • C9 deficiency with dermatomyositis antibody
      • C9D antibody
      • CO9_HUMAN antibody
      • Complement component 9 antibody
      • Complement component 9 deficiency antibody
      • Complement component C9 antibody
      • Complement component C9b antibody
      see all

    Images

    • All lanes : Anti-C9 antibody (ab67040) at 1 µg/ml

      Lane 1 : Human Plasma Total Protein Lysate
      Lane 2 : Human placenta tissue lysate - total protein (ab29745)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 63 kDa
      Observed band size: 74 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 16 minutes


      Human Complement component C9 precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 74 kDa band observed is also comparable to the molecular weight seen with other commercially available antibodies to C9.

    References

    ab67040 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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