Overview

  • Product name

  • Description

    Rabbit polyclonal to C9
  • Host species

    Rabbit
  • Specificity

    This product may also detect C9 within the C5b-9 complex, but we have no evidence to show this. Any feedback from our customers is welcome. We recommend ab55811 for specifically detecting the C5b-9 complex.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human C9 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab71329)

  • Positive control

    • Recombinant Human C9 protein (ab114199) can be used as a positive control in WB. This antibody gave a positive signal in Human Placenta Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab71330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • Sequence similarities

    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 1 TSP type-1 domain.
  • Post-translational
    modifications

    Thrombin cleaves factor C9 to produce C9a and C9b.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARMD15 antibody
    • C9 antibody
    • C9 deficiency antibody
    • C9 deficiency with dermatomyositis antibody
    • C9D antibody
    • CO9_HUMAN antibody
    • Complement component 9 antibody
    • Complement component 9 deficiency antibody
    • Complement component C9 antibody
    • Complement component C9b antibody
    see all

Images

  • Anti-C9 antibody (ab71330) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    We hypothesize that the 70 kDa band represents the glycosylated C9 protein (predicted molecular weight 63). Under appropriate conditions this antibody should also recognise the C9a cleavage product.
  • ab71330, at a 1/2000 dilution, staining human C9 in placenta tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

    See Abreview

References

This product has been referenced in:

  • Merle NS  et al. Intravascular hemolysis activates complement via cell-free heme and heme-loaded microvesicles. JCI Insight 3:N/A (2018). Read more (PubMed: 29925688) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your inquiry.

I can confirm that both antibodies may also detect C9 within the C5b-9 complex, but we have no evidence to show this. Any feedback from our customers is welcome.

Both antibodieswere raised against an immunogen from C9 (a component of the C5b-9 complex).

We recommend ab55811 for specifically detecting the C5b-9 complex.

https://www.abcam.com/index.html?datasheet=55811 (or use the following: https://www.abcam.com/index.html?datasheet=55811).

I hope this information is helpful and wish you a good weekend.

Read More

Answer

Thank you for contacting us.
After discussing this matter with my colleagues in our lab, we have decided to change the target for this antibody from C5b-9 to C9. The antibody was raised against a sequence from the C9 component of the C5b-9 complex. While it is possible that this antibody may recognize the complex as well as C9, our testing does not currently prove that explicitly. The WB band we observe corresponds to C9 because it was tested under reduced and denatured conditions. Under native conditions, it is possible that ab71330 would detect the complex at a much higher molecular weight, but this has not been tested in house.
My colleagues are currently expanding our testing for this antibody in ICC and IHC and hope to provide more information about whether or not it detects the complex in the near future. I hope this helps, please let me know if you need any additional information or assistance.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Specification
Placenta
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted May 26 2010

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