Recombinant
RabMAb

Anti-C9 antibody [EPR11232] (ab168345)

Submit an Abreview Submit a Question

Overview

  • Product name
    Anti-C9 antibody [EPR11232]
    See all C9 primary antibodies
  • Description
    Rabbit monoclonal [EPR11232] to C9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human C9.
    Database link: P02748

  • Positive control
    • Human fetal liver, Human angioneoplasm, Human placenta, Human serum and Human plasma lysates, Human lung tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11232
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab168345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
IHC-P 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function
      Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
    • Tissue specificity
      Plasma.
    • Involvement in disease
      Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    • Sequence similarities
      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 EGF-like domain.
      Contains 1 LDL-receptor class A domain.
      Contains 1 MACPF domain.
      Contains 1 TSP type-1 domain.
    • Post-translational
      modifications
      Thrombin cleaves factor C9 to produce C9a and C9b.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization
      Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
    • Information by UniProt
    • Database links
      • Alternative names
        • ARMD15 antibody
        • C9 antibody
        • C9 deficiency antibody
        • C9 deficiency with dermatomyositis antibody
        • C9D antibody
        • CO9_HUMAN antibody
        • Complement component 9 antibody
        • Complement component 9 deficiency antibody
        • Complement component C9 antibody
        • Complement component C9b antibody
        see all

      Images

      • Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345)
        Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345)

        Immunoprecipitation: Western blot analysis on immunoprecipitation pellet from Human plasma cell lysate using ab168345 at a 1/10 dilution.



        Anti-C9 antibody [EPR11232] (ab168345) at 1/10 dilution + Human plasma cell lysate
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)

        Immunohistochemical analysis of paraffin embedded Human lung tissue labeling C9 with ab168345 at a 1/100 dilution.

      • Western blot - Anti-C9 antibody [EPR11232] (ab168345)
        Western blot - Anti-C9 antibody [EPR11232] (ab168345)
        All lanes : Anti-ZNF239 antibody (ab118345) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : Human angioneoplasm lysate
        Lane 3 : Human placenta lysate
        Lane 4 : Human serum lysate
        Lane 5 : Human plasma lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 63 kDa

      References

      ab168345 has not yet been referenced specifically in any publications.

      Publishing research using ab168345? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab168345.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      Sign up