Recombinant Anti-C9 antibody [EPR11232] (ab168345)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11232] to C9
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-C9 antibody [EPR11232]
See all C9 primary antibodies -
Description
Rabbit monoclonal [EPR11232] to C9 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanIP HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human C9.
Database link: P02748 -
Positive control
- Human fetal liver, Human angioneoplasm, Human placenta, Human serum and Human plasma lysates, Human lung tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11232 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC |
Human
|
IHC-P |
Human
|
IP |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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|
IP |
1/10 - 1/100.
|
Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 63 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
Target
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Function
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. -
Sequence similarities
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 1 TSP type-1 domain. -
Post-translational
modificationsThrombin cleaves factor C9 to produce C9a and C9b.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore. - Information by UniProt
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Database links
- Entrez Gene: 735 Human
- Omim: 120940 Human
- SwissProt: P02748 Human
- Unigene: 654443 Human
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Alternative names
- ARMD15 antibody
- C9 antibody
- C9 deficiency antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)
Immunohistochemical analysis of paraffin embedded Human lung tissue labeling C9 with ab168345 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunoprecipitation: Western blot analysis on immunoprecipitation pellet from Human plasma cell lysate using ab168345 at a 1/10 dilution.
Anti-C9 antibody [EPR11232] (ab168345) at 1/10 dilution + Human plasma cell lysate -
All lanes : Anti-ZNF239 antibody (ab118345) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human angioneoplasm lysate
Lane 3 : Human placenta lysate
Lane 4 : Human serum lysate
Lane 5 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 63 kDa
Protocols
References (1)
ab168345 has been referenced in 1 publication.
- Corsetti G et al. Autophagy and Oncosis/Necroptosis Are Enhanced in Cardiomyocytes from Heart Failure Patients. Med Sci Monit Basic Res 25:33-44 (2019). PubMed: 30713336