RecombinantRabMAb

Recombinant Anti-C9 antibody [EPR11232] (ab168345)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)
  • Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345)
  • Western blot - Anti-C9 antibody [EPR11232] (ab168345)
  • Anti-C9 antibody [EPR11232] (ab168345)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11232] to C9
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-C9 antibody [EPR11232]
    See all C9 primary antibodies

  • Description

    Rabbit monoclonal [EPR11232] to C9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human C9.
    Database link: P02748

  • Positive control

    • Human fetal liver, Human angioneoplasm, Human placenta, Human serum and Human plasma lysates, Human lung tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11232

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab168345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.

    • Tissue specificity

      Plasma.

    • Involvement in disease

      Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.

    • Sequence similarities

      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 EGF-like domain.
      Contains 1 LDL-receptor class A domain.
      Contains 1 MACPF domain.
      Contains 1 TSP type-1 domain.

    • Post-translational
      modifications

      Thrombin cleaves factor C9 to produce C9a and C9b.
      Phosphorylation sites are present in the extracelllular medium.

    • Cellular localization

      Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
    • Information by UniProt

    • Database links

      • Alternative names

        • ARMD15 antibody
        • C9 antibody
        • C9 deficiency antibody
        • C9 deficiency with dermatomyositis antibody
        • C9D antibody
        • CO9_HUMAN antibody
        • Complement component 9 antibody
        • Complement component 9 deficiency antibody
        • Complement component C9 antibody
        • Complement component C9b antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)

        Immunohistochemical analysis of paraffin embedded Human lung tissue labeling C9 with ab168345 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345)
        Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345)

        Immunoprecipitation: Western blot analysis on immunoprecipitation pellet from Human plasma cell lysate using ab168345 at a 1/10 dilution.



        Anti-C9 antibody [EPR11232] (ab168345) at 1/10 dilution + Human plasma cell lysate
      • Western blot - Anti-C9 antibody [EPR11232] (ab168345)
        Western blot - Anti-C9 antibody [EPR11232] (ab168345)
        All lanes : Anti-ZNF239 antibody (ab118345) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : Human angioneoplasm lysate
        Lane 3 : Human placenta lysate
        Lane 4 : Human serum lysate
        Lane 5 : Human plasma lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 63 kDa

      • Anti-C9 antibody [EPR11232] (ab168345)
        Anti-C9 antibody [EPR11232] (ab168345)

      References (1)

      Publishing research using ab168345? Please let us know so that we can cite the reference in this datasheet.

      ab168345 has been referenced in 1 publication.

      • Corsetti G  et al. Autophagy and Oncosis/Necroptosis Are Enhanced in Cardiomyocytes from Heart Failure Patients. Med Sci Monit Basic Res 25:33-44 (2019). PubMed: 30713336

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