Recombinant Anti-C9 antibody [EPR11232] (ab168345)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)](https://www.abcam.com/ps/products/168/ab168345/Images/ab168345-169128-anti-c9-antibody-epr11232-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11232] to C9
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-C9 antibody [EPR11232]
See all C9 primary antibodies -
Description
Rabbit monoclonal [EPR11232] to C9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human C9.
Database link: P02748 -
Positive control
- Human fetal liver, Human angioneoplasm, Human placenta, Human serum and Human plasma lysates, Human lung tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11232 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/10 - 1/100.
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| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 63 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
1/10 - 1/100. |
Application notes
Is unsuitable for ICC/IF.
Target
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Function
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. -
Sequence similarities
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 1 TSP type-1 domain. -
Post-translational
modificationsThrombin cleaves factor C9 to produce C9a and C9b.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore. - Information by UniProt
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Database links
- Entrez Gene: 735 Human
- Omim: 120940 Human
- SwissProt: P02748 Human
- Unigene: 654443 Human
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Alternative names
- ARMD15 antibody
- C9 antibody
- C9 deficiency antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)
Immunohistochemical analysis of paraffin embedded Human lung tissue labeling C9 with ab168345 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345)](https://www.abcam.com/ps/products/168/ab168345/Images/ab168345-169242-anti-c9-antibody-epr11232-immunoprecipitation.jpg)
Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345)Immunoprecipitation: Western blot analysis on immunoprecipitation pellet from Human plasma cell lysate using ab168345 at a 1/10 dilution.
Anti-C9 antibody [EPR11232] (ab168345) at 1/10 dilution + Human plasma cell lysate -
![Western blot - Anti-C9 antibody [EPR11232] (ab168345)](https://www.abcam.com/ps/products/168/ab168345/Images/ab168345-169126-anti-c9-antibody-epr11232-western-blot.jpg)
Western blot - Anti-C9 antibody [EPR11232] (ab168345)All lanes : Anti-ZNF239 antibody (ab118345) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human angioneoplasm lysate
Lane 3 : Human placenta lysate
Lane 4 : Human serum lysate
Lane 5 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 63 kDa -
![Anti-C9 antibody [EPR11232] (ab168345)](https://www.abcam.com/ps/products/168/ab168345/Images/ab168345-4-benefits-of-recombinant-antibodies.png)
Anti-C9 antibody [EPR11232] (ab168345)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab168345 has been referenced in 1 publication.
- Corsetti G et al. Autophagy and Oncosis/Necroptosis Are Enhanced in Cardiomyocytes from Heart Failure Patients. Med Sci Monit Basic Res 25:33-44 (2019). PubMed: 30713336