Recombinant
RabMAb

Recombinant Anti-C9 antibody [EPR11232] (ab168345)

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Overview

  • Product name

    Anti-C9 antibody [EPR11232]
    See all C9 primary antibodies

  • Description

    Rabbit monoclonal [EPR11232] to C9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Recombinant fragment corresponding to Human C9.
    Database link: P02748

  • Positive control

    • Human fetal liver, Human angioneoplasm, Human placenta, Human serum and Human plasma lysates, Human lung tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11232

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.

    • Tissue specificity

      Plasma.

    • Involvement in disease

      Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.

    • Sequence similarities

      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 EGF-like domain.
      Contains 1 LDL-receptor class A domain.
      Contains 1 MACPF domain.
      Contains 1 TSP type-1 domain.

    • Post-translational
      modifications

      Thrombin cleaves factor C9 to produce C9a and C9b.
      Phosphorylation sites are present in the extracelllular medium.

    • Cellular localization

      Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
    • Information by UniProt

    • Database links

      • Alternative names

        • ARMD15 antibody
        • C9 antibody
        • C9 deficiency antibody
        • C9 deficiency with dermatomyositis antibody
        • C9D antibody
        • CO9_HUMAN antibody
        • Complement component 9 antibody
        • Complement component 9 deficiency antibody
        • Complement component C9 antibody
        • Complement component C9b antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (ab168345)

        Immunohistochemical analysis of paraffin embedded Human lung tissue labeling C9 with ab168345 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345)
        Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345)

        Immunoprecipitation: Western blot analysis on immunoprecipitation pellet from Human plasma cell lysate using ab168345 at a 1/10 dilution.



        Anti-C9 antibody [EPR11232] (ab168345) at 1/10 dilution + Human plasma cell lysate
      • Western blot - Anti-C9 antibody [EPR11232] (ab168345)
        Western blot - Anti-C9 antibody [EPR11232] (ab168345)
        All lanes : Anti-ZNF239 antibody (ab118345) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : Human angioneoplasm lysate
        Lane 3 : Human placenta lysate
        Lane 4 : Human serum lysate
        Lane 5 : Human plasma lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 63 kDa

      References

      This product has been referenced in:

      • Corsetti G  et al. Autophagy and Oncosis/Necroptosis Are Enhanced in Cardiomyocytes from Heart Failure Patients. Med Sci Monit Basic Res 25:33-44 (2019). Read more (PubMed: 30713336) »
      See 1 Publication for this product

      Publishing research using ab168345? Please let us know so that we can cite the reference in this datasheet.

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