Key features and details
- Rabbit polyclonal to C9orf40
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-C9orf40 antibody
DescriptionRabbit polyclonal to C9orf40
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Orangutan
Synthetic peptide within Human C9orf40 aa 75-125. The exact sequence is proprietary. NP_060468.2
Database link: Q8IXQ3
- Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH 3T3 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab202286 was affinity purified using an epitope specific to C9orf40 immobilized on solid support.
Our Abpromise guarantee covers the use of ab202286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 21 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
- Information by UniProt
- C9orf40 antibody
- Chromosome 9 open reading frame 40 antibody
- CI040_HUMAN antibody
All lanes : Anti-C9orf40 antibody (ab202286) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells
Lane 2 : Whole cell lysate from 293T cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from mouse TCMK-1 cells
Lane 5 : Whole cell lysate from mouse NIH 3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 21 kDa
Exposure time: 3 minutes
Lane 1: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognizes an epitope of C9orf40.
Lane 2: Detection of Human C9orf40 by Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202286 at 6 µg per reaction for IP.
Lane 3: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab202286 at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time
of 3 minutes.
ab202286 has not yet been referenced specifically in any publications.