Overview

  • Product name

    Anti-C9orf40 antibody
  • Description

    Rabbit polyclonal to C9orf40
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide within Human C9orf40 aa 75-125. The exact sequence is proprietary. NP_060468.2
    Database link: Q8IXQ3

  • Positive control

    • Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH 3T3 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab202286 was affinity purified using an epitope specific to C9orf40 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 21 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-C9orf40 antibody (ab202286) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells
    Lane 2 : Whole cell lysate from 293T cells
    Lane 3 : Whole cell lysate from Jurkat cells
    Lane 4 : Whole cell lysate from mouse TCMK-1 cells
    Lane 5 : Whole cell lysate from mouse NIH 3T3 cells

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 21 kDa


    Exposure time: 3 minutes
  • Lane 1: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognizes an epitope of C9orf40.
    Lane 2: Detection of Human C9orf40 by Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202286 at 6 µg per reaction for IP.
    Lane 3: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.
    Subsequent Western blot detection used ab202286 at 0.4 µg/ml.

     

    Detection: Chemiluminescence with an exposure time
    of 3 minutes.

References

ab202286 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab202286.
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