Key features and details
- Rabbit polyclonal to C9ORF46/PLGRKT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-C9ORF46/PLGRKT antibody
DescriptionRabbit polyclonal to C9ORF46/PLGRKT
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- WB: A431, NIH/3T3 and U-251 MG whole cell lysates; Mouse lung tissue lysate. IHC-P: Human colon cancer and skin tissue. ICC/IF: U-251 MG cells.
This product was previously labelled as C9ORF46
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%
Our Abpromise guarantee covers the use of ab237525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 17 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionReceptor for plasminogen. Regulates urokinase plasminogen activator-dependent and stimulates tissue-type plasminogen activator-dependent cell surface plasminogen activation. Proposed to be part of a local catecholaminergic cell plasminogen activation system that regulates neuroendocrine prohormone processing. Involved in regulation of inflammatory response; regulates monocyte chemotactic migration and matrix metallproteinase activation, such as of MMP2 and MMP9.
Tissue specificityExpressed in peripheral blood cells and monocytes. Expressed in adrenal medulla.
Cellular localizationCell membrane. Colocalizes on the cell surface with urokinase plasminogen activator surface receptor/PLAUR.
- Information by UniProt
- AD025 antibody
- Chromosome 9 open reading frame 46 antibody
- FLJ14688 antibody
All lanes : Anti-C9ORF46/PLGRKT antibody (ab237525) at 1/500 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : U-251 MG (Human brain glioma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 17 kDa
Paraffin-embedded human colon cancer tissue stained for C9ORF46/PLGRKT using ab237525 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human skin tissue stained for C9ORF46/PLGRKT using ab237525 at 1/100 dilution in immunohistochemical analysis.
U-251 MG (Human brain glioma cell line) cells stained for C9ORF46/PLGRKT (green) using ab237525 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237525 has not yet been referenced specifically in any publications.