Overview

  • Product name
    Anti-C9orf7 antibody - C-terminal
  • Description
    Rabbit polyclonal to C9orf7 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human C9orf7 aa 122-151 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9UGQ2

  • Positive control
    • Mouse stomach tissue lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab173228 is purified through a Protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • Anti-C9orf7 antibody - C-terminal (ab173228) at 1/100 dilution + Mouse stomach tissue lysate at 35 µg

    Predicted band size: 18 kDa

  • ab173228 staining C9orf7 in rat cerebellar granule neuron cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 2% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA) for 30 minutes at 25°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

ab173228 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Loading amount
370000 cells
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Sample
Rat Cell lysate - whole cell (Cerebellar granule neurons culture)
Specification
Cerebellar granule neurons culture
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 26 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Rat Cell (Cerebellar granule neurons culture)
Specification
Cerebellar granule neurons culture
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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