Product nameAnti-C9orf72 antibody
See all C9orf72 primary antibodies
DescriptionChicken polyclonal to C9orf72
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide. This information is considered to be commercially sensitive.
- ICC/IF: U-2 OS cells. IHC-P: Human cerebellum tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
- Information by UniProt
- ALSFTD antibody
- C9orf72 antibody
- chromosome 9 open reading frame 72 antibody
4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for C9orf72 (green) using ab235189 at 5 μg/ml (overnight at 4°C) in ICC/IF. Goat Anti-Chicken IgG DyLight™ 488 Preadsorbed was used as the secondary antibody 15 µg/ml (1 hour at RT). Nuclear counterstain was DAPI.
Formalin/PFA-fixed paraffin-embedded human cerebellum tissue stained for C9orf72 using ab235189 at a 1/200 dilution (30mins at RT) in immunohistochemistry analysis. Anti-Chicken-HRP secondary used at a 1/500 dilution (20mins at RT). Counterstain hemotoxylin.
Top panel: H&E 20x.
Bottom left panel: ab235189, 1/200, 20x.
Bottom right panel: ab235189, 1/200, 40x.
ab235189 has not yet been referenced specifically in any publications.