Key features and details
- Chicken polyclonal to C9orf72
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgY
Product nameAnti-C9orf72 antibody
See all C9orf72 primary antibodies
DescriptionChicken polyclonal to C9orf72
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide. This information is considered to be commercially sensitive.
- ICC/IF: U-2 OS cells. IHC-P: Human cerebellum tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
- Information by UniProt
- ALSFTD antibody
- C9orf72 antibody
- chromosome 9 open reading frame 72 antibody
4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for C9orf72 (green) using ab235189 at 5 μg/ml (overnight at 4°C) in ICC/IF. Goat Anti-Chicken IgG DyLight™ 488 Preadsorbed was used as the secondary antibody 15 µg/ml (1 hour at RT). Nuclear counterstain was DAPI.
Formalin/PFA-fixed paraffin-embedded human cerebellum tissue stained for C9orf72 using ab235189 at a 1/200 dilution (30mins at RT) in immunohistochemistry analysis. Anti-Chicken-HRP secondary used at a 1/500 dilution (20mins at RT). Counterstain hemotoxylin.
Top panel: H&E 20x.
Bottom left panel: ab235189, 1/200, 20x.
Bottom right panel: ab235189, 1/200, 40x.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235189 has not yet been referenced specifically in any publications.