Recombinant Anti-C9orf72 antibody [EPR22021] (ab221137)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22021] to C9orf72
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-C9orf72 antibody [EPR22021]
See all C9orf72 primary antibodies -
Description
Rabbit monoclonal [EPR22021] to C9orf72 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C6, Neuro-2a, SH-SY5Y, U-87 MG and PC-12 whole cell lysate. Rat, human and mouse brain lysate. Human hippocampus lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22021 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 203228 Human
- Entrez Gene: 73205 Mouse
- Entrez Gene: 313155 Rat
- Omim: 614260 Human
- SwissProt: Q96LT7 Human
- SwissProt: Q6DFW0 Mouse
- SwissProt: Q66HC3 Rat
- Unigene: 493639 Human
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Alternative names
- ALSFTD antibody
- C9orf72 antibody
- chromosome 9 open reading frame 72 antibody
see all
Images
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All lanes : Anti-C9orf72 antibody [EPR22021] (ab221137) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell line) whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-C9orf72 antibody [EPR22021] (ab221137) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : Human brain lysate
Lane 5 : Human hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab221137 has been referenced in 3 publications.
- Alvarez-Mora MI et al. Bioenergetic and Autophagic Characterization of Skin Fibroblasts from C9orf72 Patients. Antioxidants (Basel) 11:N/A (2022). PubMed: 35740026
- Butti Z et al. Reduced C9orf72 function leads to defective synaptic vesicle release and neuromuscular dysfunction in zebrafish. Commun Biol 4:792 (2021). PubMed: 34172817
- Laflamme C et al. Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Elife 8:N/A (2019). WB ; Human . PubMed: 31612854