Anti-C9orf86 antibody (ab111866)
Key features and details
- Rabbit polyclonal to C9orf86
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-C9orf86 antibody -
Description
Rabbit polyclonal to C9orf86 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-219 of Human C9orf86 (AAH02945).
-
Positive control
- 293T, A431 and Raji cells and whole cell lysates.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab111866 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab111866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 80 kDa. | |
ICC/IF | 1/100 - 1/500. |
Target
-
Function
May enhance cellular proliferation. May reduce growth inhibitory activity of CDKN2A. -
Post-translational
modificationsIsoform RBEL1A is O-glycosylated, while other isoforms are not. -
Cellular localization
Nucleus. Predominantly nuclear and Cytoplasm. Predominantly cytoplasmic. - Information by UniProt
-
Database links
- Entrez Gene: 55684 Human
- Entrez Gene: 227624 Mouse
- Entrez Gene: 227624 Rat
- Omim: 610615 Human
- SwissProt: Q3YEC7 Human
- SwissProt: Q5U3K5 Mouse
- Unigene: 370555 Human
- Unigene: 29600 Mouse
see all -
Alternative names
- bA216L13.9 antibody
- Chromosome 9 open reading frame 86 antibody
- FLJ10101 antibody
see all
Images
-
Anti-C9orf86 antibody (ab111866) at 1/5000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS-PAGE -
ab111866 at 1/200 dilution staining C9orf86 in paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower panel is costained with Hoechst 33342.
Protocols
References (1)
ab111866 has been referenced in 1 publication.
- Tang H et al. RBEL1 is required for osteosarcoma cell proliferation via inhibiting retinoblastoma 1. Mol Med Rep 13:1275-80 (2016). PubMed: 26676380