Anti-C9orf86 antibody (ab111866)

Submit a review Q&A (4)References (1)
Western blot - Anti-C9orf86 antibody (ab111866)
  • Immunocytochemistry/ Immunofluorescence - Anti-C9orf86 antibody (ab111866)

Key features and details

  • Rabbit polyclonal to C9orf86
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-C9orf86 antibody

  • Description

    Rabbit polyclonal to C9orf86

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-219 of Human C9orf86 (AAH02945).

  • Positive control

    • 293T, A431 and Raji cells and whole cell lysates.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab111866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
ICC/IF 1/100 - 1/500.

Target

  • Function

    May enhance cellular proliferation. May reduce growth inhibitory activity of CDKN2A.

  • Post-translational
    modifications

    Isoform RBEL1A is O-glycosylated, while other isoforms are not.

  • Cellular localization

    Nucleus. Predominantly nuclear and Cytoplasm. Predominantly cytoplasmic.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • bA216L13.9 antibody
    • Chromosome 9 open reading frame 86 antibody
    • FLJ10101 antibody
    • FLJ13045 antibody
    • GTP-binding protein Parf antibody
    • Hypothetical protein LOC55684 antibody
    • KIAA1984 antibody
    • OTTHUMP00000022608 antibody
    • OTTHUMP00000022610 antibody
    • OTTHUMP00000022612 antibody
    • OTTHUMP00000022613 antibody
    • OTTHUMP00000022614 antibody
    • OTTHUMP00000022615 antibody
    • PARF antibody
    • Partner of ARF antibody
    • pp8875 antibody
    • Putative GTP binding protein Parf antibody
    • Rab like GTP binding protein 1 antibody
    • Rab like GTP binding protein 1C antibody
    • Rab like protein 1 antibody
    • Rab-like protein 1 antibody
    • RABL6 antibody
    • RBEL1 antibody
    • RBEL1_HUMAN antibody
    • RP11-216L13.9 antibody
    see all

Images

  • Western blot - Anti-C9orf86 antibody (ab111866)
    Western blot - Anti-C9orf86 antibody (ab111866)
    Anti-C9orf86 antibody (ab111866) at 1/5000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 80 kDa



    7.5% SDS-PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-C9orf86 antibody (ab111866)
    Immunocytochemistry/ Immunofluorescence - Anti-C9orf86 antibody (ab111866)
    ab111866 at 1/200 dilution staining C9orf86 in paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower panel is costained with Hoechst 33342.

References (1)

Publishing research using ab111866? Please let us know so that we can cite the reference in this datasheet.

ab111866 has been referenced in 1 publication.

  • Tang H  et al. RBEL1 is required for osteosarcoma cell proliferation via inhibiting retinoblastoma 1. Mol Med Rep 13:1275-80 (2016). PubMed: 26676380

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

Thank you for sending us the replacement antibody. Unfortunately the antibody delivered today was warm to the touch and the blue ice was completely melted. As the storage buffer of the antibody did not have any glycerol in it, do you think the antibody will be suitable for use?

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Answer

Thanks for your email.

The vial of ab111866 you recevied was shipped on Saturday to arrive on Monday. We have done internal testing and determined that antibodies kept at 37 degrees C are stable for at least a week. There should be no ill effects based on the short amount of time the antibody was not cold. Please use it as you normally would.

As always, we guarantee our antibodies to work as expected based on tested applications and species noted on the online datasheets.Please contact me if you feel the antibody is not performing as expected.

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Question


Would it be possible to attain an aliquot of the polyclonal to CCDC21 (ab117782) as a replacement for the anti-C9orf86? Thank you.

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab117782.

To check the status of the order please contact our Customer Service team and reference this number. This product is currently back ordered and delivery is expected around June 28.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question



Catalog number(s): ab111866

Product(s): Rabbit polyclonal to C9orf86

Survey results
---------------------------------------------------------------------------------------------------------------------

Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. Yes
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A. Yes

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.No
Q. How did you feel about being given protocol advice?
A.
Q. Did the product work successfully after following the advice?
A.
Q. Why have you not tried the protocol advice? (Select all that apply.)
A.
A.
A.
A.

Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. Yes

Q. Did the replacement product work successfully?
A.No
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A.No

Q. Would you have preferred a different outcome from receiving a credit note or refund?
A.
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Satisfied
Q. Could we have done anything to better resolve the problem?
A.I believe the situation was handled properly.
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.The process was fair and prompt.

Read More
Answer

Thank you for taking the time to complete our survey. I am sorry to hear the replacement antibody still did not work as expected.

I am happy to offeranother anti-C9orf86 antibody, or any other primary antibody, credit or refund. Please let me know how you wish to proceed.

Please feel free to reply to this email if you would like to discuss this issue further.

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Question

I recently received ab111866. it worked the first time in wb and ICC, but then after freezing for a few weeks it did not give reproducible data on identical experiments; no bands were seen on wb as before and the subcellular localization demonstrated by this antibody changed to nuclear, which is incorrect.

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab57603.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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