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Using kit ab65356.
According to the protocol, the cell culture samples are prepared in HCl. We then need to measure the protein concentration.
Please confirm which protein assays are suitable for use with this type of acidic buffer.
Could BCA or Bradford be used?
Asked on Nov 28 2012
Thank you for your recent telephone enquiry.
I can confirm that the Bradford assay can be used for protein quantifation with this kit.
The BCA protein assay is not compatible with the acidic buffer in the samples you would have prepared with this kit.
I hope this information has been useful for you. Please do not hesitate to let me know if you have any other questions.
Abcam Scientific Support
Answered on Nov 28 2012