Key features and details
- Rabbit polyclonal to CA150
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CA150 antibody
See all CA150 primary antibodies
DescriptionRabbit polyclonal to CA150
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla
Immunogen was a synthetic peptide, which represented a portion of human transcription elongation regulator 1 (CA150) encoded within exon 1 (LocusLink ID 10915).
- Nuclear extract from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were affinity purified using the peptide immobilized on solid support.
Our Abpromise guarantee covers the use of ab10481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter.
Tissue specificityDetected in brain neurons.
Sequence similaritiesContains 6 FF domains.
Contains 3 WW domains.
DomainThe FF domains bind the phosphorylated C-terminus of the largest subunit of RNA polymerase II, probably mediate interaction with HTATSF1 and preferentially bind peptides with the consensus sequence [DE](2-5)-[FWY]-[DE](2-5).
The WW domains bind Pro-rich domains.
- Information by UniProt
- CA 150 antibody
- Co activator of 150 kDa antibody
- Coactivator of 150 kDa antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling CA150 at 1/1000 (1µg/ml). Detection: DAB.
ab10481 detection of human CA150 by Western blot. Sample: Nuclear extract (5
µg) from HeLa cells. Antibody: ab10481 used at 0.33 µg/ml. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3,000 with NBT-BCIP as substrate for 20 min.
ab10481 detection of human CA150 by Western blot. Sample: Nuclear extract (5 µg) from HeLa cells. Antibody: ab10481 used at 0.33 µg/ml. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3,000 with NB
ab10481 has not yet been referenced specifically in any publications.