Overview

  • Product name
    Anti-CA19-9 antibody [121SLE]
    See all CA19-9 primary antibodies
  • Description
    Mouse monoclonal [121SLE] to CA19-9
  • Host species
    Mouse
  • Specificity
    The antibody recognizes Sialyl Lewis a containing glycolipids.
  • Tested applications
    Suitable for: Flow Cyt, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.

  • Positive control
    • In Western Blot, this antibody gave a positive signal in human breast and human kidney tissue lysates.
  • General notes

    This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab3982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 22490524

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

ELISA Use at an assay dependent concentration.
IHC-P 1/100.
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.

Target

  • Alternative names
    • CA19.9 antibody
    • Ovarian tumor antigen antibody
    • Pancreatic tumor antigen antibody
    • Sialyl Lewis a antibody

Images

  • ab3982 staining CA19-9 in human colorectal adenocarcinoma by IHC-P (Formalin/Paraformaldehye fixed paraffin embedded tissue sections).

  • All lanes : Anti-CA19-9 antibody [121SLE] (ab3982) at 5 µg/ml

    Lane 2 : Human breast tissue lysate - total protein (ab30090)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 32 kDa
    Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

References

This product has been referenced in:
  • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). Read more (PubMed: 29343722) »
  • Gomez DL  et al. Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate. PLoS One 10:e0133862 (2015). IHC . Read more (PubMed: 26288179) »
See all 10 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Melanoma cell line
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 19 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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