Anti-CAB3 antibody (ab16717)
Key features and details
- Rabbit polyclonal to CAB3
- Suitable for: WB, IP, ICC/IF
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-CAB3 antibody -
Description
Rabbit polyclonal to CAB3 -
Host species
Rabbit -
Specificity
This antibody recognises the beta 3 calcium channel subunit. -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to CAB3 aa 1-15 conjugated to keyhole limpet haemocyanin.
Sequence:MYDDSYVPGFEDSEA
-
Positive control
- Rat cortical and hippocampal, neuronal membranes. Negative control: non neuronal cells
-
General notes
Protein previously labeled as CACNB3.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab16717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 58 kDa. | |
| IP | Use at an assay dependent dilution. | |
| ICC/IF | Use at an assay dependent dilution. |
Target
-
Function
The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. -
Tissue specificity
Expressed mostly in brain, smooth muscle and ovary. -
Sequence similarities
Belongs to the calcium channel beta subunit family.
Contains 1 SH3 domain. - Information by UniProt
-
Database links
- Entrez Gene: 25297 Rat
- SwissProt: P54287 Rat
- Unigene: 2808 Rat
-
Alternative names
- CAB3 antibody
- CACB3_HUMAN antibody
- Cacnb3 antibody
see all
Images
-
Western blot - Anti-CAB3 antibody (ab16717)Image from Uriu Y et al, J Biol Chem. 2010 Jul 9;285(28):21750-67. Epub 2010 May 7, Fig 7. DOI 10.1074/jbc.M110.101311PC12 cells transfected with siRNAs were solubilized and analyzed by WB using ab16717.
Protocols
Datasheets and documents
References
ab16717 has been referenced in 1 publication.
- Uriu Y et al. Rab3-interacting molecule gamma isoforms lacking the Rab3-binding domain induce long lasting currents but block neurotransmitter vesicle anchoring in voltage-dependent P/Q-type Ca2+ channels. J Biol Chem 285:21750-67 (2010). WB ; Human . PubMed: 20452978
